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Labeda DP,
( 2011 )
Multilocus sequence analysis of phytopathogenic species of the genus Streptomyces.
International journal of systematic and evolutionary microbiology 61 (Pt 10)
PMID : 21112986 :
DOI : 10.1099/ijs.0.028514-0
Abstract >>
The identification and classification of species within the genus Streptomyces is difficult because there are presently 576 species with validly published names and this number increases every year. The value of multilocus sequence analysis applied to the systematics of Streptomyces species has been well demonstrated in several recently published papers. In this study the sequence fragments of four housekeeping genes, atpD, recA, rpoB and trpB, were determined for the type strains of 10 known phytopathogenic species of the genus Streptomyces, including Streptomyces scabiei, Streptomyces acidiscabies, Streptomyces europaeiscabiei, Streptomyces luridiscabiei, Streptomyces niveiscabiei, Streptomyces puniciscabiei, Streptomyces reticuliscabiei, Streptomyces stelliscabiei, Streptomyces turgidiscabies and Streptomyces ipomoeae, as well as six uncharacterized phytopathogenic Streptomyces isolates. The type strains of 52 other species, including 19 species observed to be phylogenetically closely related to these, based on 16S rRNA gene sequence analysis, were also included in the study. Phylogenetic analysis of single gene alignments and a concatenated four-gene alignment demonstrated that the phytopathogenic species are taxonomically distinct from each other in spite of high 16S rRNA gene sequence similarities and provided a tool for the identification of unknown putative phytopathogenic Streptomyces strains at the species level.
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Pet?í?ková K,
Chro?áková A,
Zelenka T,
Chrudimský T,
Pospíšil S,
Pet?í?ek M,
Krištůfek V,
( 2015 )
Evolution of cyclizing 5-aminolevulinate synthases in the biosynthesis of actinomycete secondary metabolites: outcomes for genetic screening techniques.
Frontiers in microbiology 6 (N/A)
PMID : 26300877 :
DOI : 10.3389/fmicb.2015.00814
PMC : PMC4525017
Abstract >>
A combined approach, comprising PCR screening and genome mining, was used to unravel the diversity and phylogeny of genes encoding 5-aminolevulinic acid synthases (ALASs, hemA gene products) in streptomycetes-related strains. In actinomycetes, these genes were believed to be directly connected with the production of secondary metabolites carrying the C5N unit, 2-amino-3-hydroxycyclopent-2-enone, with biological activities making them attractive for future use in medicine and agriculture. Unlike "classical" primary metabolism ALAS, the C5N unit-forming cyclizing ALAS (cALAS) catalyses intramolecular cyclization of nascent 5-aminolevulinate. Specific amino acid sequence changes can be traced by comparison of "classical" ALASs against cALASs. PCR screening revealed 226 hemA gene-carrying strains from 1,500 tested, with 87% putatively encoding cALAS. Phylogenetic analysis of the hemA homologs revealed strain clustering according to putative type of metabolic product, which could be used to select producers of specific C5N compound classes. Supporting information was acquired through analysis of actinomycete genomic sequence data available in GenBank and further genetic or metabolic characterization of selected strains. Comparison of 16S rRNA taxonomic identification and BOX-PCR profiles provided evidence for numerous horizontal gene transfers of biosynthetic genes or gene clusters within actinomycete populations and even from non-actinomycete organisms. Our results underline the importance of environmental and evolutionary data in the design of efficient techniques for identification of novel producers.
Keyword | MeSH Terms |
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
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