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1. Pet?í?ková  K, Chro?áková  A, Zelenka  T, Chrudimský  T, Pospíšil  S, Pet?í?ek  M, Krištůfek  V,     ( 2015 )

Evolution of cyclizing 5-aminolevulinate synthases in the biosynthesis of actinomycete secondary metabolites: outcomes for genetic screening techniques.

Frontiers in microbiology 6 (N/A)
PMID : 26300877  :   DOI  :   10.3389/fmicb.2015.00814     PMC  :   PMC4525017    
Abstract >>
A combined approach, comprising PCR screening and genome mining, was used to unravel the diversity and phylogeny of genes encoding 5-aminolevulinic acid synthases (ALASs, hemA gene products) in streptomycetes-related strains. In actinomycetes, these genes were believed to be directly connected with the production of secondary metabolites carrying the C5N unit, 2-amino-3-hydroxycyclopent-2-enone, with biological activities making them attractive for future use in medicine and agriculture. Unlike "classical" primary metabolism ALAS, the C5N unit-forming cyclizing ALAS (cALAS) catalyses intramolecular cyclization of nascent 5-aminolevulinate. Specific amino acid sequence changes can be traced by comparison of "classical" ALASs against cALASs. PCR screening revealed 226 hemA gene-carrying strains from 1,500 tested, with 87% putatively encoding cALAS. Phylogenetic analysis of the hemA homologs revealed strain clustering according to putative type of metabolic product, which could be used to select producers of specific C5N compound classes. Supporting information was acquired through analysis of actinomycete genomic sequence data available in GenBank and further genetic or metabolic characterization of selected strains. Comparison of 16S rRNA taxonomic identification and BOX-PCR profiles provided evidence for numerous horizontal gene transfers of biosynthetic genes or gene clusters within actinomycete populations and even from non-actinomycete organisms. Our results underline the importance of environmental and evolutionary data in the design of efficient techniques for identification of novel producers.
KeywordMeSH Terms
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
2. Han  JH, Cho  MH, Kim  SB,     ( 2012 )

Ribosomal and protein coding gene based multigene phylogeny on the family Streptomycetaceae.

Systematic and applied microbiology 35 (1)
PMID : 22154623  :   DOI  :   10.1016/j.syapm.2011.08.007    
Abstract >>
The phylogenetic relationship among the three genera of the family Streptomycetaceae was examined using the small and large subunit ribosomal RNA genes, and the gyrB, rpoB, trpB, atpD and recA genes. The total stretches of the analyzed ribosomal genes were 4.2kb, and those of five protein coding genes were 4.5 kb. The resultant phylogenetic trees confirmed that each genus formed an independent clade in the majority of cases. The G+C contents of rRNA genes were 56.9-58.9 mol%, and those of protein coding genes were 65.4-72.4 mol%, the latter being closer to those of the genomic DNAs. The average nucleotide sequence identity between the organisms were 94.1-96.4% for rRNA genes and 85.7-90.6% for protein coding genes, thus indicating that protein coding genes can give higher resolution than rRNA genes. In addition, the protein coding gene trees were more stable than the rRNA gene trees, supported by higher bootstrap values and other treeing algorithms. Moreover, the genome data of six Streptomyces species indicated that many protein coding genes exhibited higher correlations with genome relatedness. The combined gene sequences were also shown to give a better resolution with higher stability than any single genes, though not necessarily more correlated with genome relatedness. It is evident from this study that the rRNA gene based phylogeny can be misleading, and also that protein coding genes have a number of advantages over the rRNA genes as the phylogenetic markers including a high correlation with the genome relatedness.
KeywordMeSH Terms
Genes, rRNA
Phylogeny

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