Home / BCRC Content / 16959 / 

Return

  Research Article

The information shown in this page was generated using the cross-referenced linkage within public domain database between their strains and BCRC related strains. Usually the information provided from public domain databases varies with diffent confidences and errors, BCRC provides the related information here at best effort, but BCRC doesn't take the responsibility about the correctness of the information provided here.

1. Pet?í?ková  K, Chro?áková  A, Zelenka  T, Chrudimský  T, Pospíšil  S, Pet?í?ek  M, Krištůfek  V,     ( 2015 )

Evolution of cyclizing 5-aminolevulinate synthases in the biosynthesis of actinomycete secondary metabolites: outcomes for genetic screening techniques.

Frontiers in microbiology 6 (N/A)
PMID : 26300877  :   DOI  :   10.3389/fmicb.2015.00814     PMC  :   PMC4525017    
Abstract >>
A combined approach, comprising PCR screening and genome mining, was used to unravel the diversity and phylogeny of genes encoding 5-aminolevulinic acid synthases (ALASs, hemA gene products) in streptomycetes-related strains. In actinomycetes, these genes were believed to be directly connected with the production of secondary metabolites carrying the C5N unit, 2-amino-3-hydroxycyclopent-2-enone, with biological activities making them attractive for future use in medicine and agriculture. Unlike "classical" primary metabolism ALAS, the C5N unit-forming cyclizing ALAS (cALAS) catalyses intramolecular cyclization of nascent 5-aminolevulinate. Specific amino acid sequence changes can be traced by comparison of "classical" ALASs against cALASs. PCR screening revealed 226 hemA gene-carrying strains from 1,500 tested, with 87% putatively encoding cALAS. Phylogenetic analysis of the hemA homologs revealed strain clustering according to putative type of metabolic product, which could be used to select producers of specific C5N compound classes. Supporting information was acquired through analysis of actinomycete genomic sequence data available in GenBank and further genetic or metabolic characterization of selected strains. Comparison of 16S rRNA taxonomic identification and BOX-PCR profiles provided evidence for numerous horizontal gene transfers of biosynthetic genes or gene clusters within actinomycete populations and even from non-actinomycete organisms. Our results underline the importance of environmental and evolutionary data in the design of efficient techniques for identification of novel producers.
KeywordMeSH Terms
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites
5-aminolevulinate synthase
C5N unit
Streptomyces
gene evolution
genetic screening
horizontal gene transfer
secondary metabolites

331, Shih-Pin Rd., Hsinchu 30062, Taiwan

Phone: +886-3-5223191

E-mail: bcrcweb@firdi.org.tw

web maintainance: +886-3-5223191 ext 593

Copyright © 2018.BCRC All rights reserved.The duplication or use of information and data such as texts or images or any linkage the website at the "bcrc.firdi.org.tw" is only permitted with the indication of the source or with prior approval by the BCRC(Bioresource Collection and Research Center).