1. |
Chao SH,
Sasamoto M,
Kudo Y,
Fujimoto J,
Tsai YC,
Watanabe K,
( 2010 ) Lactobacillus odoratitofui sp. nov., isolated from stinky tofu brine. PMID : 20097799 : DOI : 10.1099/ijs.0.019307-0 DOI : 10.1099/ijs.0.019307-0 Abstract >>
Three Gram-positive-staining strains isolated from fermented stinky tofu brine were rod-shaped, non-motile, asporogenous, facultatively anaerobic, heterofermentative and did not exhibit catalase activity. Comparative analyses of 16S rRNA, rpoA and pheS gene sequences demonstrated that the novel strains were members of the genus Lactobacillus. On the basis of 16S rRNA gene sequence similarity, the type strains of Lactobacillus collinoides (98.6 %), Lactobacillus paracollinoides (98.6 %) and Lactobacillus similis (99.6 %) were the closest neighbours. However, DNA-DNA reassociation values with these strains were less than 10 %. The phenotypic and genotypic features demonstrated that these isolates represent a novel species of the genus Lactobacillus, for which the name Lactobacillus odoratitofui sp. nov. is proposed. The type strain is YIT 11304(T) (=JCM 15043(T) =BCRC 17810(T) =DSM 19909(T)).
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2. |
Sun Z,
Harris HM,
McCann A,
Guo C,
Argimón S,
Zhang W,
Yang X,
Jeffery IB,
Cooney JC,
Kagawa TF,
Liu W,
Song Y,
Salvetti E,
Wrobel A,
Rasinkangas P,
Parkhill J,
Rea MC,
O'Sullivan O,
Ritari J,
Douillard FP,
Paul Ross R,
Yang R,
Briner AE,
Felis GE,
de Vos WM,
Barrangou R,
Klaenhammer TR,
Caufield PW,
Cui Y,
Zhang H,
O'Toole PW,
( 2015 ) Expanding the biotechnology potential of lactobacilli through comparative genomics of 213 strains and associated genera. PMID : 26415554 : DOI : 10.1038/ncomms9322 PMC : PMC4667430 Abstract >>
Lactobacilli are a diverse group of species that occupy diverse nutrient-rich niches associated with humans, animals, plants and food. They are used widely in biotechnology and food preservation, and are being explored as therapeutics. Exploiting lactobacilli has been complicated by metabolic diversity, unclear species identity and uncertain relationships between them and other commercially important lactic acid bacteria. The capacity for biotransformations catalysed by lactobacilli is an untapped biotechnology resource. Here we report the genome sequences of 213 Lactobacillus strains and associated genera, and their encoded genetic catalogue for modifying carbohydrates and proteins. In addition, we describe broad and diverse presence of novel CRISPR-Cas immune systems in lactobacilli that may be exploited for genome editing. We rationalize the phylogenomic distribution of host interaction factors and bacteriocins that affect their natural and industrial environments, and mechanisms to withstand stress during technological processes. We present a robust phylogenomic framework of existing species and for classifying new species.
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