| 1. |
Szwalbe AJ,
Williams K,
Song Z,
de Mattos-Shipley K,
Vincent JL,
Bailey AM,
Willis CL,
Cox RJ,
Simpson TJ,
( 2019 ) Characterisation of the biosynthetic pathway to agnestins A and B reveals the reductive route to chrysophanol in fungi. PMID : 30746079 : DOI : 10.1039/c8sc03778g PMC : PMC6335632 Abstract >>
Two new dihydroxy-xanthone metabolites, agnestins A and B, were isolated from Paecilomyces variotii along with a number of related benzophenones and xanthones including monodictyphenone. The structures were elucidated by NMR analyses and X-ray crystallography. The agnestin (agn) biosynthetic gene cluster was identified and targeted gene disruptions of the PKS, Baeyer-Villiger monooxygenase, and other oxido-reductase genes revealed new details of fungal xanthone biosynthesis. In particular, identification of a reductase responsible for in vivo anthraquinone to anthrol conversion confirms a previously postulated essential step in aromatic deoxygenation of anthraquinones, e.g. emodin to chrysophanol.
|
2. |
Williams K,
Szwalbe AJ,
Dickson C,
Desson TR,
Mulholland NP,
Vincent JL,
Clough JM,
Bailey AM,
Butts CP,
Willis CL,
Simpson TJ,
Cox RJ,
( 2017 ) Genetic and chemical characterisation of the cornexistin pathway provides further insight into maleidride biosynthesis. PMID : 28660939 : DOI : 10.1039/c7cc03303f Abstract >>
The biosynthesis of the herbicide cornexistin in the fungus Paecilomyces variotii was investigated by full sequencing of its genome, knockout of key genes within its biosynthetic gene cluster and isolation and identification of intermediate compounds. The general biosynthetic pathway resembles that of byssochlamic acid and other nonadrides in the early stages, but differs in requiring fewer enzymes in the key nonadride dimerisation step, and in the removal of one maleic anhydride moiety.
|
331, Shih-Pin Rd., Hsinchu 30062, Taiwan
Phone: +886-3-5223191
E-mail: bcrcweb@firdi.org.tw
web maintainance: +886-3-5223191 ext 593
Copyright © 2018.BCRC All rights reserved.The duplication or use of information and data such as texts or images or any linkage the website at the "bcrc.firdi.org.tw" is only permitted with the indication of the source or with prior approval by the BCRC(Bioresource Collection and Research Center).