Ait Tayeb L,
( N/A )
Molecular phylogeny of the genus Pseudomonas based on rpoB sequences and application for the identification of isolates.
PMID : 15950132 : DOI : 10.1016/j.resmic.2005.02.009
Phylogenetic relationships within the genus Pseudomonas were examined by comparing partial (about 1000 nucleotides) rpoB gene sequences. A total of 186 strains belonging to 75 species of Pseudomonas sensu stricto and related species were studied. The phylogenetic resolution of the rpoB tree was approximately three times higher than that of the rrs tree. Ribogroups published earlier correlated well with rpoB sequence clusters. The rpoB sequence database generated by this study was used for identification. A total of 89 isolates (79.5%) were identified to a named species, while 16 isolates (14.3%) corresponded to unnamed species, and 7 isolates (6.2%) had uncertain affiliation. rpoB sequencing is now being used for routine identification of Pseudomonas isolates in our laboratory.
( 2008 )
Pseudomonas xiamenensis sp. nov., a denitrifying bacterium isolated from activated sludge.
PMID : 18676479 : DOI : 10.1099/ijs.0.65459-0
A taxonomic study was carried out on strain C10-2T, a moderately halophilic denitrifier isolated from activated sludge samples collected in China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain C10-2T belonged to the genus Pseudomonas, with highest sequence similarity of 98.1 and 97.7 % to 'Pseudomonas denitrificans' IAM 12023 and Pseudomonas pertucinogena JCM 11590T, respectively. The gyrB, rpoD and rpoB gene sequence similarity between strain C10-2T and P. pertucinogena JCM 11590T was respectively 84.1, 79.0 and 88.2 %. Phylogenetic trees based on these housekeeping genes showed that strain C10-2T and P. pertucinogena JCM 11590T form a clade at the periphery of the genus Pseudomonas. The DNA-DNA hybridization value between strain C10-2T and P. pertucinogena JCM 11590T was 30-32 %. The G+C content of the chromosomal DNA was 61.2 mol%. The combined genotypic and phenotypic data show that strain C10-2T represents a novel species of the genus Pseudomonas, for which the name Pseudomonas xiamenensis sp. nov. is proposed, with the type strain C10-2T (=CGMCC 1.6446 =JCM 13530T =MCCC 1A00089T).