Home / BCRC Content / 80569 / 

Return

  Research Article

The information shown in this page was generated using the cross-referenced linkage within public domain database between their strains and BCRC related strains. Usually the information provided from public domain databases varies with diffent confidences and errors, BCRC provides the related information here at best effort, but BCRC doesn't take the responsibility about the correctness of the information provided here.

1. Maroniche  GA, García  JE, Salcedo  F, Creus  CM,     ( 2017 )

Molecular identification of Azospirillum spp.: Limitations of 16S rRNA and qualities of rpoD as genetic markers.

Microbiological research 195 (N/A)
PMID : 28024520  :   DOI  :   10.1016/j.micres.2016.11.009    
Abstract >>
Since their discovery, plant-growth promoting rhizobacteria from the genus Azospirillum have been subjected to intensive research due to their biotechnological potential as crop inoculants. Phylogenetic analysis of Azospirillum spp. is carried out by 16S rRNA sequencing almost exclusively, but inconsistencies and low confidence often arise when working with close species. In this work, it was observed that these difficulties might be explained by a high number of rRNA operons with considerable inter-genic variability within Azospirillum genomes. To search for alternative genetic markers from a list of housekeeping genes, the correlation between pairwise gene and whole-genome similarities was examined. Due to its good performance, rpoD was selected for further analyses. Genus-specific primers for the PCR-amplification and sequencing of rpoD from Azospirillum spp. were designed and tested on 16 type strains of different species. The sequences obtained were used for inferring a phylogenetic tree of the genus, which was in turn used as a reference to successfully identify a collection of 31 azospirilla isolated from many different locations of Argentine. In addition, several strains that might represent novel species were detected. The results indicate that the sequencing of rpoD is a suitable alternative method for a confident molecular identification in Azospirillum spp.
KeywordMeSH Terms
Genome similarity
Genotyping
Phylogeny
Rhizobacteria
Genome similarity
Genotyping
Phylogeny
Rhizobacteria
Genome similarity
Genotyping
Phylogeny
Rhizobacteria
Genome similarity
Genotyping
Phylogeny
Rhizobacteria
Genome similarity
Genotyping
Phylogeny
Rhizobacteria
Genome similarity
Genotyping
Phylogeny
Rhizobacteria
Genome similarity
Genotyping
Phylogeny
Rhizobacteria
Genome similarity
Genotyping
Phylogeny
Rhizobacteria
Genetic Markers
Genotyping Techniques
2. Lin  SY, Hameed  A, Liu  YC, Hsu  YH, Lai  WA, Shen  FT, Young  CC,     ( 2015 )

Azospirillum soli sp. nov., a nitrogen-fixing species isolated from agricultural soil.

International journal of systematic and evolutionary microbiology 65 (12)
PMID : 26382036  :   DOI  :   10.1099/ijsem.0.000618     DOI  :   10.1099/ijsem.0.000618    
Abstract >>
An aerobic, Gram-stain-negative, rod or spiral-shaped diazotrophic bacterium (designated strain CC-LY788T), was isolated from agricultural soil in Taiwan. Strain CC-LY788T was able to grow at 25-40 �XC, pH 6.0-8.0 and tolerated NaCl to 2.0% (w/v). Positive for nitrogen fixation with the activity recorded as 6.5 nmol ethylene h(-1). Strain CC-LY788T showed highest 16S rRNA gene sequence similarity to Azospirillum picis DSM 19922T (97.2%) and Azospirillum rugosum DSM 19657T (97.1%) and lower sequence similarities (<96.6%) to all other species of the genus Azospirillum. According to the DNA-DNA hybridization, the relatedness values of strain CC-LY788T with A. picis DSM 19922T and A. rugosum DSM 19657T were 51.1��5.5% and 46.8��2.1%, respectively. Strain CC-LY788T was positive for the rapid identification of the genus-specific primer set. The respiratory quinone system was ubiquinone (Q-10) and the DNAG+C content was 69.8 mol%. The major fatty acids found in strain CC-LY788T were C16 : 0, C18 : 1 2-OH, C14 : 0 3-OH/C16 : 1 iso I (summed feature 2), C16 : 1�s7c/C16 : 1�s6c (summed feature 3), C18 : 0 ante/C18 : 2�s6,9c (summed feature 5) and C18 : 1�s7c/C18 : 1�s6c (summed feature 8). Based on the phylogenetic, phenotypic and chemotaxonomic features, strain CC-LY788T represents a novel species of the genus Azospirillum, for which the name Azospirillum soli sp. nov. is proposed. The type strain is CC-LY788T (=BCRC 80569T=JCM 18820T).
KeywordMeSH Terms
Nitrogen Fixation
Phylogeny
Soil Microbiology
Nitrogen Fixation
Phylogeny
Soil Microbiology

331, Shih-Pin Rd., Hsinchu 30062, Taiwan

Phone: +886-3-5223191

E-mail: bcrcweb@firdi.org.tw

web maintainance: +886-3-5223191 ext 593

Copyright © 2018.BCRC All rights reserved.The duplication or use of information and data such as texts or images or any linkage the website at the "bcrc.firdi.org.tw" is only permitted with the indication of the source or with prior approval by the BCRC(Bioresource Collection and Research Center).