| 1. |
Hiraga K,
Ueno Y,
Sukontasing S,
Tanasupawat S,
Oda K,
( 2008 ) Lactobacillus senmaizukei sp. nov., isolated from Japanese pickle. PMID : 18599706 : DOI : 10.1099/ijs.0.65677-0 DOI : 10.1099/ijs.0.65677-0 Abstract >>
The taxonomic position of a lactic acid bacterium, strain L13(T), isolated from senmaizuke, a Japanese traditional pickle, was studied. This strain was a heterofermentative, facultatively anaerobic, Gram-positive, non-spore-forming, non-motile, rod-shaped bacterium. It produced dl-lactic acid from glucose. Phylogenetic analysis of its 16S rRNA gene sequence and physiological and biochemical characteristics indicated that the strain was a member of the genus Lactobacillus. Based on 16S rRNA gene sequence similarity, strain L13(T) was closely related to Lactobacillus hammesii CIP 108387(T) (98.9 %) and Lactobacillus parabrevis ATCC 53295(T) (98.5 %). In addition, the rpoA gene sequence of strain L13(T) was closely related to those of L. hammesii CIP 108387(T) (92.2 %) and L. parabrevis ATCC 53295(T) (92.0 %). However, DNA-DNA hybridization of strain L13(T) with these two strains revealed that strain L13(T) represents a separate genomic species. The DNA G+C content of strain L13(T) was 46 mol% and the peptidoglycan was of the A4alpha l-Lys-d-Asp type. Thus, these data indicate that strain L13(T) represents a novel species of the genus Lactobacillus, for which the name Lactobacillus senmaizukei is proposed. The type strain is L13(T) (=NBRC 103853(T)=TISTR 1847(T)).
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2. |
Sun Z,
Harris HM,
McCann A,
Guo C,
Argimón S,
Zhang W,
Yang X,
Jeffery IB,
Cooney JC,
Kagawa TF,
Liu W,
Song Y,
Salvetti E,
Wrobel A,
Rasinkangas P,
Parkhill J,
Rea MC,
O'Sullivan O,
Ritari J,
Douillard FP,
Paul Ross R,
Yang R,
Briner AE,
Felis GE,
de Vos WM,
Barrangou R,
Klaenhammer TR,
Caufield PW,
Cui Y,
Zhang H,
O'Toole PW,
( 2015 ) Expanding the biotechnology potential of lactobacilli through comparative genomics of 213 strains and associated genera. PMID : 26415554 : DOI : 10.1038/ncomms9322 PMC : PMC4667430 Abstract >>
Lactobacilli are a diverse group of species that occupy diverse nutrient-rich niches associated with humans, animals, plants and food. They are used widely in biotechnology and food preservation, and are being explored as therapeutics. Exploiting lactobacilli has been complicated by metabolic diversity, unclear species identity and uncertain relationships between them and other commercially important lactic acid bacteria. The capacity for biotransformations catalysed by lactobacilli is an untapped biotechnology resource. Here we report the genome sequences of 213 Lactobacillus strains and associated genera, and their encoded genetic catalogue for modifying carbohydrates and proteins. In addition, we describe broad and diverse presence of novel CRISPR-Cas immune systems in lactobacilli that may be exploited for genome editing. We rationalize the phylogenomic distribution of host interaction factors and bacteriocins that affect their natural and industrial environments, and mechanisms to withstand stress during technological processes. We present a robust phylogenomic framework of existing species and for classifying new species.
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