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Hiraga K,
Ueno Y,
Sukontasing S,
Tanasupawat S,
Oda K,
( 2008 ) Lactobacillus senmaizukei sp. nov., isolated from Japanese pickle. PMID : 18599706 : DOI : 10.1099/ijs.0.65677-0 Abstract >>
The taxonomic position of a lactic acid bacterium, strain L13(T), isolated from senmaizuke, a Japanese traditional pickle, was studied. This strain was a heterofermentative, facultatively anaerobic, Gram-positive, non-spore-forming, non-motile, rod-shaped bacterium. It produced dl-lactic acid from glucose. Phylogenetic analysis of its 16S rRNA gene sequence and physiological and biochemical characteristics indicated that the strain was a member of the genus Lactobacillus. Based on 16S rRNA gene sequence similarity, strain L13(T) was closely related to Lactobacillus hammesii CIP 108387(T) (98.9 %) and Lactobacillus parabrevis ATCC 53295(T) (98.5 %). In addition, the rpoA gene sequence of strain L13(T) was closely related to those of L. hammesii CIP 108387(T) (92.2 %) and L. parabrevis ATCC 53295(T) (92.0 %). However, DNA-DNA hybridization of strain L13(T) with these two strains revealed that strain L13(T) represents a separate genomic species. The DNA G+C content of strain L13(T) was 46 mol% and the peptidoglycan was of the A4alpha l-Lys-d-Asp type. Thus, these data indicate that strain L13(T) represents a novel species of the genus Lactobacillus, for which the name Lactobacillus senmaizukei is proposed. The type strain is L13(T) (=NBRC 103853(T)=TISTR 1847(T)).
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2. |
Naser SM,
Dawyndt P,
Hoste B,
Gevers D,
Vandemeulebroecke K,
Cleenwerck I,
Vancanneyt M,
Swings J,
( 2007 ) Identification of lactobacilli by pheS and rpoA gene sequence analyses. PMID : 18048724 : DOI : 10.1099/ijs.0.64711-0 Abstract >>
The aim of this study was to evaluate the use of the phenylalanyl-tRNA synthase alpha subunit (pheS) and the RNA polymerase alpha subunit (rpoA) partial gene sequences for species identification of members of the genus Lactobacillus. Two hundred and one strains representing the 98 species and 17 subspecies were examined. The pheS gene sequence analysis provided an interspecies gap, which in most cases exceeded 10 % divergence, and an intraspecies variation of up to 3 %. The rpoA gene sequences revealed a somewhat lower resolution, with an interspecies gap normally exceeding 5 % and an intraspecies variation of up to 2 %. The combined use of pheS and rpoA gene sequences offers a reliable identification system for nearly all species of the genus Lactobacillus. The pheS and rpoA gene sequences provide a powerful tool for the detection of potential novel Lactobacillus species and synonymous taxa. In conclusion, the pheS and rpoA gene sequences can be used as alternative genomic markers to 16S rRNA gene sequences and have a higher discriminatory power for reliable identification of species of the genus Lactobacillus.
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