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Taxonomy Citation ID Reference
3104 Tsukamura  M,     ( 1982 )

Numerical analysis of the taxonomy of Nocardiae and Rhodococci.

Microbiology and immunology 26 (12)
PMID : 6763136 DOI  :   10.1111/j.1348-0421.1982.tb00260.x    
Abstract >>
The genera Nocardia and Rhodococcus were clearly differentiated in the present study. Eleven characteristics were shown to be useful for differentiation between these two genera. Nocardia asteroides sunsu stricto previously defined by Tsukamura was divided into two taxa. One contained the type strain and was considered to retain the name Nocardia asteroides in a new sense. Another was named in the present study as Nocardia nova sp. nov. Tsukamura. The type strain of this species is ATCC 33726. The following seven characters were useful for differentiating N. nova from newly defined N. asteroides: 1) arylsulfatase activity after 14 days; 2) catalase activity (semiquantitative); 3) beta-esterase activity; 4) pyrazinamidase activity; 5) utilization of citrate as a sole source of carbon; 6) utilization of 2,3-butylene glycol as a sole carbon source; and 7) resistance to 5-fluorouracil (20 micrograms/ml). The name Nocardia farcinica for Tsukamura's Kyoto-I group should be rejected. This taxon has been named Nocardia paratuberculosis sp. nov. Tsukamura. The type strain is ATCC 23826. Three new species of the genus Rhodococcus were proposed: Rhodococcus aichiensis sp. nov. Tsukamura (type strain, ATCC 33611); Rhodococcus chubuensis sp. nov. Tsukamura (type strain, ATCC 33609); Rhodococcus obuensis sp. nov. Tsukamura (type strain, ATCC 33610).
KeywordMeSH Terms
55450 Tamura  T, Ohji  S, Ichikawa  N, Hosoyama  A, Yamazoe  A, Hamada  M, Komaki  H, Shibata  C, Matsuzawa  T, Gonoi  T, Suzuki  KI, Fujita  N,     ( 2018 )

Reclassification of Nocardia species based on whole genome sequence and associated phenotypic data.

The Journal of antibiotics 71 (7)
PMID : 29618770 DOI  :   10.1038/s41429-018-0043-1    
Abstract >>
Type strains of 72 validated Nocardia species were phylogenetically analyzed based on the multilocus sequence analysis (MLSA) concatenated atpD-groL1-groL2-recA-rpoA-secY-sodA-ychF. Furthermore, their similarity based on digital DNA-DNA hybridization (dDDH) was calculated. Nocardia soli, Nocardia cummidelens and Nocardia salmonicida, Nocardia nova and Nocardia elegans, Nocardia exalbida and Nocardia gamkensis, and Nocardia coubleae and Nocardia ignorata formed coherent clades, respectively. Moreover, each set showed over 70% relatedness by dDDH and shared common phenotypic characteristics. Therefore, we propose a reclassification of Nocardia soli and Nocardia cummidelens as a later heterotypic synonym of Nocardia salmonicida, Nocardia elegans as a later heterotypic synonym of Nocardia nova, Nocardia gamkensis as a later heterotypic synonym of Nocardia exalbida, and Nocardia coubleae as a later heterotypic synonym of Nocardia ignorata.
KeywordMeSH Terms
2905 VALIDATION LIST No. 12. Int. J. Syst. Bacteriol. (1983) 33:896-897.

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