|Taxonomy Citation ID||Reference|
( 2017 )
Description of Conyzicola nivalis sp. nov., isolated from glacial snow, and emended description of the genus Conyzicola and Conyzicola lurida.
PMID : 28820090 DOI : 10.1099/ijsem.0.002027
A cold-tolerant, translucent, yellow-pigmented, Gram-stain-positive, non-motile, rod-shaped bacteria was isolated from snow of the Zadang Glacier on the Tibetan Plateau, PR China. 16S rRNA gene sequence similarity analysis indicated that the isolate was closely related to Conyzicola lurida KCTC 29231T and Leifsonia psychrotolerans DSM 22824T at a level of 97.72 and 97.49 %, respectively. Other close relatives had a 16S rRNA gene sequence similarity of less than 97 %. The major cell-wall amino acid was 2,4-diaminobutyric acid. The cell-wall sugars were rhamnose, ribose, glucose, galactose and mannose. The major menaquinones were MK-9, MK-10 and MK-11. The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The predominant fatty acids were anteiso-C15 : 0 and iso-C16 : 0. In DNA-DNA hybridization tests, strain ZD5-4T shared 43.6 and 34.2 % relatedness with C. lurida KCTC 29231T and L. psychrotolerans DSM 22824T, respectively. Based on phenotypic and chemotaxonomic characteristics, strain ZD5-4T was considered to represent a novel species of the genus Conyzicola, for which the name Conyzicola nivalis sp. nov. is proposed. The type strain is ZD5-4T (=JCM 30076T=CGMCC 1.12813T). Emended descriptions of the genus Conyzicola and Conyzicola lurida are also provided.
( 2014 )
Conyzicola lurida gen. nov., sp. nov., isolated from the root of Conyza canadensis.
PMID : 24860113 DOI : 10.1099/ijs.0.056754-0
A novel Gram-stain-positive, non-spore-forming, pale yellow, irregular rod-shaped bacterium designated strain HWE2-01(T) was isolated from the surface-sterilized root of horseweed (Conyza canadensis). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain HWE2-01(T) belongs to the family Microbacteriaceae and showed sequence similarity levels of 97.1-97.7% with species of the genus Salinibacterium, 95.9-97.6% with species of the genus Leifsonia and 97.1% with Homoserinimonas aerilata. The highest sequence similarity (97.7%) was with Salinibacterium xinjiangense 0543(T). The genomic DNA G+C content of the novel strain was 68.1 mol%. The predominant cellular fatty acid of strain HWE2-01(T) was anteiso-C15 : 0, major menaquinones were MK-10, MK-9 and MK-11, and diagnostic polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The peptidoglycan of the novel strain contained 2,4-diaminobutyric acid, alanine, glycine and glutamic acid. The cell-wall sugars of strain HWE2-01(T) were galactose, mannose and rhamnose. The murein was of the acetyl type. Based on the results of the phenotypic and phylogenetic analysis, strain HWE2-01(T) differed in some respects from other members of the family Microbacteriaceae. Therefore, strain HWE2-01(T) is proposed to represent a novel species of a new genus in the family Microbacteriaceae with the name Conyzicola lurida gen. nov., sp. nov. (type strain = HWE2-01(T) = KCTC 29231(T) = JCM 19257(T)).