|Taxonomy Citation ID||Reference|
( 2007 )
Flavobacterium terrae sp. nov. and Flavobacterium cucumis sp. nov., isolated from greenhouse soil.
PMID : 17625200 DOI : 10.1099/ijs.0.64935-0
Two bacterial strains, R2A1-13(T) and R2A45-3(T), were isolated from greenhouse soils in Korea. The cells of both strains were Gram-negative, aerobic and rod-shaped. 16S rRNA gene sequence analysis placed the isolates in the genus Flavobacterium within the family Flavobacteriaceae. Strain R2A1-13(T) was found to be related to Flavobacterium columnare IAM 14301(T), Flavobacterium saliperosum CGMCC1.3801(T) and Flavobacterium croceum EMB47(T), with sequence similarities of 96.8, 95.0 and 94.6 %, respectively. Strain R2A45-3(T) was found to be related to F. croceum EMB47(T) and Flavobacterium aquatile ATCC 11947(T), with sequence similarities of 94.7 and 94.6 %, respectively. Both strains contained iso-C(15 : 0) and iso-C(16 : 0) as the main fatty acids and contained a menaquinone with six isoprene units (MK-6) as the major isoprenoid quinone. The G+C contents of the DNA from strains R2A1-13(T) and R2A45-3(T) were 34 and 38 mol%, respectively. A polyphasic taxonomic study revealed that these strains belong to two novel species within the genus Flavobacterium, for which the names Flavobacterium terrae sp. nov. and Flavobacterium cucumis sp. nov. are proposed. The type strains of F. terrae sp. nov. and F. cucumis sp. nov. are R2A1-13(T) (=KACC 11731(T)=DSM 18829(T)) and R2A45-3(T) (=KACC 11732(T)=DSM 18830(T)), respectively.
( 2013 )
Flavobacterium squillarum sp. nov., isolated from a freshwater shrimp culture pond, and emended descriptions of Flavobacterium haoranii, Flavobacterium cauense, Flavobacterium terrae and Flavobacterium aquatile.
PMID : 23148098 DOI : 10.1099/ijs.0.046425-0
A bacterial strain, designated CMJ-5(T), was isolated from a freshwater shrimp culture pond in Taiwan and characterized using a polyphasic taxonomic approach. Cells of strain CMJ-5(T) were Gram-stain-negative, strictly aerobic, non-motile, yellow-pigmented rods surrounded by a thick capsule. Growth occurred at 20-35 �XC (optimum, 30 �XC), at pH 6.5-8.0 (optimum, pH 7.0) and with 0-0.5% NaCl (optimum, 0%). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain CMJ-5(T) belonged to the genus Flavobacterium and was related most closely to Flavobacterium haoranii LQY-7(T) with sequence similarity of 94.4%. Strain CMJ-5(T) contained iso-C15:0 (37.5%), iso-C17:0 3-OH (13.4%), iso-C15:0 3-OH (13.1%) and iso-C15:1 G (11.2%) as the predominant fatty acids. The major isoprenoid quinone was MK-6. The polar lipid profile consisted of phosphatidylethanolamine and several uncharacterized aminophospholipids and phospholipids. The major polyamine was homospermidine. The G+C content of the genomic DNA was 42.4 mol%. On the basis of phylogenetic inference and phenotypic data, strain CMJ-5(T) should be classified as a representative of a novel species, for which the name Flavobacterium squillarum sp. nov. is proposed. The type strain is CMJ-5(T) (=BCRC 80405(T)=LMG 26890(T)=KCTC 23915(T)). Emended descriptions of Flavobacterium haoranii, Flavobacterium cauense, Flavobacterium terrae and Flavobacterium aquatile are also proposed.