Taxonomy Citation ID | Reference | ||||
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38080 |
Chen WM,
Lin YS,
Young CC,
Sheu SY,
( 2013 ) Pseudorhodoferax aquiterrae sp. nov., isolated from groundwater. PMID : 22368172 DOI : 10.1099/ijs.0.039842-0 Abstract >>
A Gram-negative, rod-shaped, motile, non-spore-forming and poly-�]-hydroxybutyrate-containing bacterial strain, designated NAFc-7(T), was isolated from groundwater in Taiwan and was characterized using a polyphasic taxonomic approach. Growth occurred at 15-40 �XC (optimum, 30 �XC), at pH 6.0-9.0 (optimum, pH 6.0-7.0) and with 0-3 % NaCl (optimum, 0-1 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain NAFc-7(T), together with Pseudorhodoferax soli TBEA3(T) (98.2 % sequence similarity) and Pseudorhodoferax caeni SB1(T) (98.0 %), formed a deep line within the family Comamonadaceae. Strain NAFc-7(T) contained summed feature 3 (comprising C(16 : 1)�s7c and/or C(16 : 1)�s6c), C(16 : 0) and C(17 : 0) cyclo as the predominant fatty acids. The major 3-hydroxy fatty acid was C(10 : 0) 3-OH. The major isoprenoid quinone was Q-8 and the DNA G+C content was 67.6 mol%. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylserine and an uncharacterized phospholipid. The DNA-DNA relatedness of strain NAFc-7(T) with respect to recognized species of the genus Pseudorhodoferax was less than 70 %. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain NAFc-7(T) represents a novel member of the genus Pseudorhodoferax, for which the name Pseudorhodoferax aquiterrae sp. nov. is proposed. The type strain is NAFc-7(T) (= BCRC 80210(T) = LMG 26333(T) = KCTC 23314(T)).
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