A bacterial strain, designated Ruye-71(T), was isolated from a freshwater tilapiine cichlid fish culture pond in Taiwan and characterized in a taxonomic study using a polyphasic approach. Cells of strain Ruye-71(T) were Gram-stain-negative, strictly aerobic, yellow-pigmented rods that were motile by gliding. Growth occurred at 10-30 �XC (optimum, 15-30 �XC), at pH 7.0-8.0 (optimum, pH 8.0) and with 0-2 % NaCl (optimum, 0-1 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain Ruye-71(T) belonged to the genus Flavobacterium and was most closely related to Flavobacterium defluvii EMB117(T), with a 16S rRNA gene sequence similarity of 97.7 %. Strain Ruye-71(T) contained iso-C15 : 0, summed feature 3 (comprising C16 : 1�s6c and/or C16 : 1�s7c), C16 : 0, C16 : 0 3-OH, iso-C15 : 0 3-OH and iso-C17 : 0 3-OH as major fatty acids. The major isoprenoid quinone was MK-6. The polar lipid profile consisted of phosphatidylethanolamine and several unidentified polar lipids. The DNA G+C content of the genomic DNA of strain Ruye-71(T) was 39.2 mol%. The mean level of DNA-DNA relatedness between strain Ruye-71(T) and Flavobacterium defluvii DSM 17963(T) was 39.9��1.2 %. On the basis of phylogenetic inference and phenotypic data, strain Ruye-71(T) should be classified as representing a novel species, for which the name Flavobacterium tilapiae sp. nov. is proposed. The type strain is Ruye-71(T) (= BCRC 80262(T) = KCTC 23312(T)). Emended descriptions of Flavobacterium defluvii and Flavobacterium johnsoniae are also proposed.

We report here the draft whole-genome sequences for 18 Flavobacterium species type strains that have historically been associated with fish gill disease.

A strictly aerobic, Gram-negative, yellow-pigmented, non-spore-forming rod, designated 15C3(T), was isolated from aerobic leaf-and-branch compost at EXPO Park in Osaka, Japan. Growth was observed at 9-33 �XC (optimum 25 �XC) and pH 5.6-7.9 (optimum pH 6.1-7.0). No growth occurred with >2% (w/v) NaCl. Strain 15C3(T) reduced nitrate to nitrogen and showed catalase activity but not oxidase activity. The predominant fatty acids were iso-C(15:0), iso-C(17:0) 3-OH and summed feature 3 (comprising C(16:1)�s7c and/or iso-C(15:0) 2-OH). The isolate contained phosphatidylethanolamine as the major polar lipid and menaquinone-6 as the major respiratory quinone. The G+C content of the genomic DNA of strain 15C3(T) was 33.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 15C3(T) belonged to the genus Flavobacterium and was most closely related to Flavobacterium hercynium WB 4.2-33(T) (96.9% sequence similarity). On the basis of phenotypic and phylogenetic distinctiveness, strain 15C3(T) is considered to represent a novel species in the genus Flavobacterium, for which the name Flavobacterium compostarboris sp. nov. is proposed. The type strain is 15C3(T) (= KACC 14224(T) = JCM 16527(T)). Emended descriptions of F. hercynium, Flavobacterium resistens and Flavobacterium johnsoniae are also given.

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