Taxonomy Citation ID | Reference | ||||
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8980 |
Nogi Y,
Takami H,
( 1999 ) Reidentification of the keratinase-producing facultatively alkaliphilic Bacillus sp. AH-101 as Bacillus halodurans. PMID : 10591021 Abstract >>
Alkaliphilic Bacillus sp. AH-101 was characterized in terms of physiological and biochemical characteristics, and 16S rDNA sequence homology and DNA-DNA hybridization analyses were performed. Phylogenetic analysis of strain AH-101 based on comparison of 16S rDNA sequences revealed that this strain is closely related to Bacillus halodurans. DNA-DNA hybridization of AH-101 and related Bacillus reference strains showed that the highest level of DNA-DNA relatedness (88%) was found between strain AH-101 and the B. halodurans type strain (DSM497). Our findings demonstrate that strain AH-101 is a member of the species B. halodurans.
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9020 | Boyer, E.E., Ingle, M.B., and Mercer, G.D. "Bacillus alcalophilus subsp. halodurans subsp. nov.: an alkaline amylase producing alkalophilic organism." Int. J. Syst. Bacteriol. (1973) 23:238-242. [No PubMed record available.] | ||||
3013 | VALIDATION LIST No. 55. Int. J. Syst. Bacteriol. (1995) 45:879-880. | ||||
1385 | Nielsen, P., Fritze, D., and Priest, E.G. "Phenetic diversity of alkaliphilic Bacillus strains: proposal for nine new species." Microbiology (1995) 141:1745-1761. [No PubMed record available.] | 11197 |
Martínez MA,
Delgado OD,
Breccia JD,
Baigorí MD,
Siñeriz F,
( 2002 ) Revision of the taxonomic position of the xylanolytic Bacillus sp. MIR32 reidentified as Bacillus halodurans and plasmid-mediated transformation of B. halodurans. PMID : 12382115 DOI : 10.1007/s00792-002-0269-4 DOI : 10.1007/s00792-002-0269-4 Abstract >>
Bacillus sp. MIR32 has been isolated using xylan as the only carbon source, and one of its xylanolytic enzymes has been extensively studied. Biochemical analysis first related this strain to Bacillus amyloliquefaciens, but further studies based on a comparison of 16S rDNA sequences, G+C content, and DNA-DNA hybridization showed that strain MIR32 should be classified as a member of the species Bacillus halodurans. This change is also supported by the typical phenotype observed and by the results of PCR amplification directed toward spacers in rDNA and tDNA genes, which were assayed and compared with those of B. halodurans DSM 497(T). Although among alkaliphilic bacilli competence development has not been experimentally demonstrated, in this work both B. halodurans MIR32 and DSM 497(T) were transformed according to a simple procedure developed in our laboratory, reaching 10(2)-10(3) stable transformants per microgram of plasmid DNA.
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