| Taxonomy Citation ID | Reference | ||||
|---|---|---|---|---|---|
| 3451 |
Antón J,
Oren A,
Benlloch S,
Rodríguez-Valera F,
Amann R,
Rosselló-Mora R,
( 2002 ) Salinibacter ruber gen. nov., sp. nov., a novel, extremely halophilic member of the Bacteria from saltern crystallizer ponds. PMID : 11931160 DOI : 10.1099/00207713-52-2-485 Abstract >>
Five brightly red-pigmented, motile, rod-shaped, extremely halophilic bacteria were isolated from saltern crystallizer ponds in Alicante (two strains) and Mallorca (three strains), Spain. They grew optimally at salt concentrations between 20 and 30% and did not grow below 15% salts. Thus, these isolates are among the most halophilic organisms known within the domain Bacteria. The temperature optimum was 37-47 degrees C. A single, yet to be identified pigment was present, with an absorption maximum at 482 nm and a shoulder at 506-510 nm. The G+C content of the DNA was 66.3-67.7 mol% and, together, they formed a homogeneous genomic group with DNA-DNA similarities above 70%. The 16S rRNA gene sequences were almost identical to sequences recovered earlier from the saltern biomass by amplification of bacterial small-subunit rRNA genes from DNA extracted from the environment. This phylotype, earlier described as 'Candidatus Salinibacter', was shown by fluorescence in situ hybridization to contribute between 5 and 25% of the prokaryote community of the saltern crystallizers. We have therefore succeeded in isolating a bacterium from the natural environment that, although being a major component of the community, was previously known by its phylotype only. Isolation of the organism now allows formal description of a novel genus and species, for which we propose the name Salinibacter ruber gen. nov., sp. nov. The type strain is strain M31T (= DSM 13855T = CECT 5946T).
|
28414 |
Makhdoumi-Kakhki A,
Amoozegar MA,
Ventosa A,
( 2012 ) Salinibacter iranicus sp. nov. and Salinibacter luteus sp. nov., isolated from a salt lake, and emended descriptions of the genus Salinibacter and of Salinibacter ruber. PMID : 21856978 DOI : 10.1099/ijs.0.031971-0 Abstract >>
Two Gram-staining-negative, red- and orange-pigmented, non-motile, rod-shaped, extremely halophilic bacteria, designated strains CB7(T) and DGO(T), were isolated from Aran-Bidgol salt lake, Iran. Growth occurred at NaCl concentrations of between 2 and 5 M NaCl and the isolates grew optimally with 3 M NaCl. The optimum pH and temperature for growth of the two strains were pH 7.5 and 37 �XC, and they were able to grow over pH and temperature ranges of pH 6-8 and 25-50 �XC. The predominant fatty acids of the two isolates were C(18:1)�s7c, iso-C(15:0) and summed feature 3 (C(16:1)�s7c and/or iso-C(15:0) 2-OH). The polar lipid pattern of the two isolates consisted of diphosphatidylglycerol, phosphatidylcholine, three unidentified lipids, one unidentified aminolipid and three unidentified glycolipids. The only quinone present was menaquinone 7 (MK-7). The G+C contents of the genomic DNA of strains CB7(T) and DGO(T) were 64.8 and 65.6 mol%, respectively. 16S rRNA gene sequence analysis indicated that strains CB7(T) and DGO(T) were related to Salinibacter ruber in the phylum Bacteroidetes. Levels of 16S rRNA gene sequence similarity between strains CB7(T) and DGO(T) and Salinibacter ruber DSM 13855(T) were 93.2 and 93.6%, respectively. The two novel strains shared 98.9% 16S rRNA gene sequence similarity. DNA-DNA hybridization experiments between strains CB7(T) and DGO(T) and Salinibacter ruber DSM 13855(T) indicated levels of relatedness of 44 and 52%, respectively, while the level of relatedness between the two new isolates was 53%. Chemotaxonomic data supported the placement of strains CB7(T) and DGO(T) in the genus Salinibacter. DNA-DNA hybridization studies and biochemical and physiological characterization allowed strains CB7(T) and DGO(T) to be differentiated from Salinibacter ruber and from each other. They are therefore considered to represent two novel species of the genus Salinibacter, for which the names Salinibacter iranicus sp. nov. (type strain CB7(T)=IBRC-M 10036(T)=CGMCC 1.11003(T)) and Salinibacter luteus sp. nov. (type strain DGO(T)=IBRC-M 10423(T)=CGMCC 1.11002(T)) are proposed. Emended descriptions of the genus Salinibacter and of Salinibacter ruber are also presented.
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