Taxonomy Citation ID | Reference | ||||
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43376 |
Kim M,
Kang O,
Zhang Y,
Ren L,
Chang X,
Jiang F,
Fang C,
Zheng C,
Peng F,
( 2016 ) Sphingoaurantiacus polygranulatus gen. nov., sp. nov., isolated from high-Arctic tundra soil, and emended descriptions of the genera Sandarakinorhabdus, Polymorphobacter and Rhizorhabdus and the species Sandarakinorhabdus limnophila, Rhizorhabdus argentea and Sphingomonas wittichii. PMID : 26475309 DOI : 10.1099/ijsem.0.000677 Abstract >>
An orange, Gram-reaction-negative and aerobic bacterium, designated MC 3718T, was isolated from a tundra soil near Ny-?lesund, Svalbard archipelago, Norway (78�X N). The cells were motile with either a polar or a subpolar flagellum and reproduced by budding or asymmetrical cell division. Growth occurred at 4-37 �XC (optimum 28-30 �XC) and at pH 6.0-10.0 (optimum pH 9.0). Many cells accumulated poly-�]-hydroxybutyrate granules and contained a single large polyphosphate granule at a pole or in the middle of the cell. Cell walls contained meso-diaminopimelic acid as the diagnostic diamino acid, and ubiquinone 10 was the main respiratory quinone. Strain MC 3718T contained summed feature 3 (comprising C16 : 1�s7c and/or C16 : 1�s6c; 29.49 %), summed feature 8 (C18 : 1�s7c and/or C18 : 1�s6c; 29.38 %), C17 : 1�s6c (10.15 %), C14 : 0 2-OH (9.05 %) and C16 : 0 (6.84 %) as the major cellular fatty acids. The main polar lipids were two sphingoglycolipids, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, three unknown phospholipids and two unknown polar lipids. Carotenoids were detected. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain MC 3718T belonged to the family Sphingomonadaceae. The DNA G+C content was 67.2 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain MC 3718T is considered to represent a novel genus and species in the family Sphingomonadaceae, for which the name Sphingoaurantiacus polygranulatus gen. nov., sp. nov. is proposed. The type strain of Sphingoaurantiacus polygranulatus is MC 3718T (= CCTCC AB 2014274T = LMG 28636T). Emended descriptions of the genera Sandarakinorhabdus, Polymorphobacter and Rhizorhabdus and the species Sandarakinorhabdus limnophila, Rhizorhabdus argentea and Sphingomonas wittichii are also provided.
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7295 |
Yabuuchi E,
Yamamoto H,
Terakubo S,
Okamura N,
Naka T,
Fujiwara N,
Kobayashi K,
Kosako Y,
Hiraishi A,
( 2001 ) Proposal of Sphingomonas wittichii sp. nov. for strain RW1T, known as a dibenzo-p-dioxin metabolizer. PMID : 11321072 DOI : 10.1099/00207713-51-2-281 Abstract >>
A polyphasic taxonomic study was performed on Sphingomonas sp. strain RW1T. The organism was isolated from water of the River Elbe and has been known as a potent metabolizer of dibenzo-p-dioxin and its relatives. TLC of a mild alkaline hydrolysate of extractable cellular lipids of strain RW1T and type strains of 21 Sphingomonas species gave a spot of sphingoglycolipid (SGL)-1 (glucuronosyl ceramide), which is characteristic of sphingomonads. In addition, strain RW1T and type strains of three Sphingomonas species (Sphingomonas yanoikuyae, Sphingomonas terrae and Sphingomonas macrogoltabidus) showed a second spot of SGL (SGL-1') identified as galacturonosyl ceramide. The presence of SGL-1 in cellular lipids suggested that strain RW1T is a member of the genus Sphingomonas. DNA-DNA reassociation rates between strain RW1T and each type strain of 14 Sphingomonas species including Sphingomonas paucimobilis, type species for the genus, revealed that strain RW1T is independent from these species. Results of phylogenetic analysis of 16S rDNA sequences of strain RW1T and type strains of 21 named Sphingomonas species verified that strain RW1T belongs to the genus Sphingomonas. Strain RW1T could be differentiated from named species of the genus by phenotypic characteristics and has been assigned to a new species, Sphingomonas wittichii sp. nov. The type strain is DSM 6014T (= JCM 10273T = EY 4224T). DNA G+C content is 67 mol %.
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