| Taxonomy Citation ID | Reference | ||||
|---|---|---|---|---|---|
| 12523 |
Ennahar S,
Cai Y,
( 2004 ) Genetic evidence that Weissella kimchii Choi et al. 2002 is a later heterotypic synonym of Weissella cibaria Bj?rkroth et al. 2002. PMID : 15023961 DOI : 10.1099/ijs.0.02783-0 Abstract >>
The taxonomic positions of Weissella kimchii and Weissella cibaria were examined using phenotypic tests, 16S rRNA gene sequencing and DNA-DNA hybridization. The two species displayed very similar sugar-fermentation patterns and produced the D-form of lactic acid. Phylogenetic analysis showed that W. cibaria is the closest neighbour of W. kimchii, sharing about 99.7 % similarity in the 16S rRNA gene sequence. DNA-DNA hybridization experiments further reinforced these results, demonstrating a 98.2 % reassociation level between W. cibaria LMG 17699(T) and W. kimchii DSM 14295(T). On the other hand, strain RO5, an isolate from paddy rice silage, exhibited 99.4 % 16S rRNA gene sequence similarity and a DNA reassociation level of more than 97 % with both type strains. Therefore, it is proposed that the species W. kimchii should be reclassified as W. cibaria.
|
3861 |
Choi HJ,
Cheigh CI,
Kim SB,
Lee JC,
Lee DW,
Choi SW,
Park JM,
Pyun YR,
( 2002 ) Weissella kimchii sp. nov., a novel lactic acid bacterium from kimchi. PMID : 11931163 DOI : 10.1099/00207713-52-2-507 Abstract >>
A gram-positive, catalase-negative, non-sporulating, facultatively anaerobic, short rod-shaped bacterium, with cells measuring 0.3-0.5 x 1-2 microm and designated strain CHJ3T, was isolated from partially fermented kimchi, a traditional Korean fermented vegetable food. The strain produced CO2 gas, D-lactate from glucose and dextran from sucrose and hydrolysed aesculin and arginine. It also fermented N-acetylglucosamine, amygdalin, arbutin, cellobiose, D-fructose, galactose, beta-gentiobiose, gluconate, D-glucose, maltose, D-mannose, salicin, sucrose and D-xylose. The G+C content of the DNA was 48.2 mol%. Phylogenetic analysis of 16S rRNA showed that strain CHJ3T is a member of the genus Weissella. The nearest phylogenetic relative of strain CHJ3T was Weissella confusa, with 16S rRNA similarity of 98.3%. However, strain CHJ3T could be differentiated from W. confusa on the basis of some phenotypic characteristics, analysis of whole-cell protein patterns and DNA-DNA hybridization data. These data suggest that strain CHJ3T be classified in the genus Weissella as a novel species, Weissella kimchii sp. nov. The type strain is CHJ3T (= KCCM 41287T = DSM 14295T = KCTC 3746T).
|
2667 |
Björkroth KJ,
Schillinger U,
Geisen R,
Weiss N,
Hoste B,
Holzapfel WH,
Korkeala HJ,
Vandamme P,
( 2002 ) Taxonomic study of Weissella confusa and description of Weissella cibaria sp. nov., detected in food and clinical samples. PMID : 11837296 DOI : 10.1099/00207713-52-1-141 Abstract >>
A taxonomic study was conducted to clarify the relationships of two bacterial populations belonging to the genus Weissella. A total of 39 strains originating mainly from Malaysian foods (22 strains) and clinical samples from humans (9 strains) and animals (6 strains) were analysed using a polyphasic taxonomic approach. The methods included classical phenotyping, whole-cell protein electrophoresis, 16S and 23S rDNA RFLP (ribotyping), determination of 16S rDNA sequence homologies and DNA-DNA reassociation levels. Based on the results, the strains were considered to represent two different species, Weissella confusa and a novel Weissella species, for which the name Weissella cibaria sp. nov. is proposed. Weisella confusa possessed the highest 16S rDNA sequence similarity to Weisella cibaria, but the DNA-DNA reassociation experiment showed hybridization levels below 49% between the strains studied. The numerical analyses of Weisella confusa and Weisella cibaria strains did not reveal any specific clustering with respect to the origin of the strains. Based on whole-cell protein electrophoresis, and ClaI and HindIII ribotyping patterns, food and clinical isolates were randomly located in the two species-specific clusters obtained.
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