Taxonomy Citation ID | Reference | ||||
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40795 |
Hameed A,
Shahina M,
Lin SY,
Liu YC,
Young CC,
( 2014 ) Pseudomonas hussainii sp. nov., isolated from droppings of a seashore bird, and emended descriptions of Pseudomonas pohangensis, Pseudomonas benzenivorans and Pseudomonas segetis. PMID : 24744016 DOI : 10.1099/ijs.0.060319-0 Abstract >>
Two Gram-staining-negative, aerobic, rod-shaped, non-spore-forming bacterial strains that are motile by a monopolar flagellum, designated CC-AMH-11(T) and CC-AMHZ-5, were isolated from droppings of a seashore bird off the coast of Hualien, Taiwan. The strains showed 99.7% mutual pairwise 16S rRNA gene sequence similarity, while exhibiting <96.2% sequence similarity to strains of other species of the genus Pseudomonas (95.7-95.9% similarity with type species, Pseudomonas aeruginosa LMG 1242T), and formed a distinct co-phyletic lineage in the phylogenetic trees. The common major fatty acids (>5% of the total) were C18 : 1�s7c and/or C18 : 1�s6c (summed feature 8), C16 : 1�s6c and/or C16 : 1�s7c (summed feature 3), C16 : 0 and C12 : 0. Phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylserine, an unidentified lipid and an unidentified phospholipid were detected as common polar lipids. The DNA G+C contents of strains CC-AMH-11(T) and CC-AMHZ-5 were 61.1 and 61.6 mol%, respectively. The common major respiratory quinone was ubiquinone 9 (Q-9), and the predominant polyamine was putrescine. The DNA-DNA hybridization obtained between the two strains was 79.0% (reciprocal value 89.4% using CC-AMHZ-5 DNA as the probe). The very high 16S rRNA gene sequence similarity and DNA-DNA relatedness and the poorly distinguishable phenotypic features witnessed between CC-AMH-11(T) and CC-AMHZ-5 suggested unambiguously that they are two distinct strains of a single genomic species. However, the strains also showed several genotypic and phenotypic characteristics that distinguished them from other closely related species of Pseudomonas. Thus, the strains are proposed to represent a novel species of Pseudomonas, for which the name Pseudomonas hussainii sp. nov. is proposed. The type strain is CC-AMH-11(T) (= JCM 19513(T) = BCRC 80696(T)); a second strain of the same species is CC-AMHZ-5 (= JCM 19512 = BCRC 80697). In addition, emended descriptions of the species Pseudomonas pohangensis, Pseudomonas benzenivorans and Pseudomonas segetis are also proposed.
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33427 | Validation List No. 145: "List of new names and new combinations previously effectively, but not validly, published." Int. J. Syst. Evol. Microbiol. (2012) 62:1017-1019. | 22350 |
Lang E,
Burghartz M,
Spring S,
Swiderski J,
Spröer C,
( 2010 ) Pseudomonas benzenivorans sp. nov. and Pseudomonas saponiphila sp. nov., represented by xenobiotics degrading type strains. PMID : 19771475 DOI : 10.1007/s00284-009-9507-7 Abstract >>
Two strains of gram-negative bacteria isolated because of their abilities to decompose xenobiotic compounds were subjected to a polyphasic taxonomic study. On the basis of 16S rRNA gene sequence analysis, the two strains were found to belong to the genus Pseudomonas. Benzene degrading strain DSM 8628(T) was moderately related to P. flavescens NCPP 3063(T) (98.3% similarity), P. monteilii CIP 104883(T), and P. plecoglossicida FPC 951(T) (98.1%). Strain DSM 9751(T) capable to grow with cetyltrimethylammonium chloride as the sole carbon source showed the highest similarity values with P. tremae CFBP 2341(T) and P. meliae MAFF 301463(T) (98.0%), both related to Pseudomonas syringae. The fatty acid pattern of strain DSM 8628(T) was distinct from patterns of other members of the genus Pseudomonas in combining a high ratio of 3OH-C(12:1) (5.1%), a low ratio of 2OH-C(12:0) (0.2%) and a relatively low ratio of C(18:1)omega7c (23.8%). On the basis of phylogenetic analysis, physiological properties and the composition of whole cell fatty acids, two novel species, Pseudomonas benzenivorans sp. nov. with the type strain DSM 8628(T) (=CIP 109857(T)) and Pseudomonas saponiphila sp. nov. with the type strain DSM 9751(T) (=CIP 109856(T)), are proposed.
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