Taxonomy Citation ID | Reference | ||||
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44522 |
Lin SY,
Hameed A,
Hung MH,
Liu YC,
Hsu YH,
Young LS,
Young CC,
( 2015 ) Pseudomonas matsuisoli sp. nov., isolated from a soil sample. PMID : 25525124 DOI : 10.1099/ijs.0.000035 DOI : 10.1099/ijs.0.000035 Abstract >>
An aerobic, Gram-stain-negative, rod-shaped and polar-flagellated bacterium, designated strain CC-MHH0089(T), was isolated from a soil sample taken on Matsu Island (Taiwan). Strain CC-MHH0089(T) grew at 15-30 �XC and pH 5.0-10.0 and tolerated ?8 % (w/v) NaCl. 16S rRNA gene sequence analysis showed high pairwise sequence similarity to Pseudomonas azotifigens 6H33b(T) (97.3 %) and Pseudomonas balearica SP1402(T) (96.7 %) and lower sequence similarity to other strains (<96.0 %). In DNA-DNA reassociation experiments, the relatedness of strain CC-MHH0089(T) to P. azotifigens JCM 12708(T) was 38.3 % (reciprocal value 19.5 %). Evolutionary trees reconstructed on the basis of 16S rRNA, gyrB and rpoB gene sequences revealed a varying phylogenetic neighbourhood of strain CC-MHH0089(T) with regard to the most closely related type strains. The predominant quinone system was ubiquinone 9 (Q-9) and the DNA G+C content was 63.6 mol%. The major fatty acids were C12 : 0, C16 : 0, C17 : 0, C19 : 0 cyclo �s8c and summed features 2 (C14 : 0 3-OH/iso-C16 : 1 I), 3 (C16 : 1�s7c/C16 : 1�s6c) and 8 (C18 : 1�s7c/C18 : 1�s6c). The major polar lipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine and diphosphatidylglycerol. According to its distinct phylogenetic, phenotypic and chemotaxonomic features, strain CC-MHH0089(T) is proposed to represent a novel species within the genus Pseudomonas, for which the name Pseudomonas matsuisoli sp. nov. is proposed. The type strain is CC-MHH0089(T) (= BCRC 80771(T) = JCM 30078(T)).
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