| Taxonomy Citation ID | Reference | ||||
|---|---|---|---|---|---|
| 22514 |
Ryu SH,
Park M,
Jeon Y,
Lee JR,
Park W,
Jeon CO,
( 2007 ) Flavobacterium filum sp. nov., isolated from a wastewater treatment plant in Korea. PMID : 17766867 DOI : 10.1099/ijs.0.65138-0 Abstract >>
A Gram-negative bacterium, designated strain EMB34(T), was isolated from a wastewater treatment plant in Korea. Growth was observed between 10 and 40 degrees C (optimum, 25-35 degrees C) and between pH 6.0 and 9.5 (optimum, pH 7.5-8.0). The cells were non-motile rods, linked with extracellular fibrils. The predominant fatty acids of strain EMB34(T) were iso-C(15 : 0), C(15 : 0), iso-C(15 : 1) G, iso-C(16 : 0) 3-OH and iso-C(15 : 0) 3-OH and the strain contained phosphatidylethanolamine and phosphatidylinositol as the polar lipids. The G+C content of the genomic DNA was 34.2 mol% and the major quinone was menaquinone-6. Comparative 16S rRNA gene sequence analyses showed that strain EMB34(T) formed a distinct phyletic line within the genus Flavobacterium. The levels of 16S rRNA gene sequence similarity with other Flavobacterium species were less than 94.5 %. On the basis of phenotypic, chemotaxonomic and molecular data, it is clear that strain EMB34(T) represents a novel species within the genus Flavobacterium, for which the name Flavobacterium filum sp. nov. is proposed. The type strain is EMB34(T) (=KCTC 12610(T)=DSM 17961(T)).
|
43624 |
Li A,
Liu H,
Sun B,
Zhou Y,
Xin Y,
( 2014 ) Flavobacterium lacus sp. nov., isolated from a high-altitude lake, and emended description of Flavobacterium filum. PMID : 24425814 DOI : 10.1099/ijs.0.056689-0 Abstract >>
Two Gram-stain-negative, non-motile, rod-shaped bacterial strains, designated NP180(T) and NR80, were isolated from water of Nam Co Lake, located in Tibet, China. Growth of strains NP180(T) and NR80 occurred at 4-25 �XC and at pH 6.5-10.0 (optima, 15-20 �XC and pH 7.5-8.5). The 16S rRNA gene sequence similarity to the phylogenetically closest related strains, Flavobacterium filum EMB 34(T), F. ponti GSW-R14(T) and F. gelidilacus LMG 21477(T), was 95.1, 94.8 and 94.6 %, respectively. The predominant fatty acids were iso-C15 : 0, iso-C15 : 1 G, iso-C17 : 0 3-OH and summed feature 9 (comprising iso-C17 : 1�s9c and/or 10-methyl C16 : 0). The major menaquinone of the two strains was menaquinone 6 (MK-6). Phosphatidylethanolamine, one unidentified aminolipid and one unidentified lipid were the major polar lipids in both strains. The G+C contents of the genomic DNA were 34.9 and 35.1 mol%, respectively, for strains NP180(T) and NR80. DNA-DNA relatedness between strains NP180(T) and NR80 was 99 %, indicating that they belong to the same species. According to phylogenetic inference and phenotypic characteristics, a novel species, Flavobacterium lacus sp. nov., is proposed. The type strain is NP180(T) (= CGMCC 1.12504(T) = NBRC 109715(T)). An emended description of Flavobacterium filum is also provided.
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