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Taxonomy Citation ID Reference
39845 Yoshida  S, Hiraga  K, Takehana  T, Taniguchi  I, Yamaji  H, Maeda  Y, Toyohara  K, Miyamoto  K, Kimura  Y, Oda  K,     ( 2016 )

A bacterium that degrades and assimilates poly(ethylene terephthalate).

Science (New York, N.Y.) 351 (6278)
PMID : 26965627 DOI  :   10.1126/science.aad6359     DOI  :   10.1126/science.aad6359    
Abstract >>
Poly(ethylene terephthalate) (PET) is used extensively worldwide in plastic products, and its accumulation in the environment has become a global concern. Because the ability to enzymatically degrade PET has been thought to be limited to a few fungal species, biodegradation is not yet a viable remediation or recycling strategy. By screening natural microbial communities exposed to PET in the environment, we isolated a novel bacterium, Ideonella sakaiensis 201-F6, that is able to use PET as its major energy and carbon source. When grown on PET, this strain produces two enzymes capable of hydrolyzing PET and the reaction intermediate, mono(2-hydroxyethyl) terephthalic acid. Both enzymes are required to enzymatically convert PET efficiently into its two environmentally benign monomers, terephthalic acid and ethylene glycol.
KeywordMeSH Terms
45823 Tanasupawat  S, Takehana  T, Yoshida  S, Hiraga  K, Oda  K,     ( 2016 )

Ideonella sakaiensis sp. nov., isolated from a microbial consortium that degrades poly(ethylene terephthalate).

International journal of systematic and evolutionary microbiology 66 (8)
PMID : 27045688 DOI  :   10.1099/ijsem.0.001058    
Abstract >>
A Gram-stain-negative, aerobic, non-spore-forming, rod-shaped bacterium, designed strain 201-F6T, was isolated from a microbial consortium that degrades poly(ethylene terephthalate) (PET) collected in Sakai city, Japan, and was characterized on the basis of a polyphasic taxonomic study. The cells were motile with a polar flagellum. The strain contained cytochrome oxidase and catalase. It grew within the pH range 5.5-9.0 (optimally at pH 7-7.5) and at 15-42 ?C (optimally at 30-37 ?C). The major isoprenoid quinone was ubiquinone with eight isoprene units (Q-8). C16 : 0, C17 : 0 cyclo, C18 :1�s7c and C12 : 0 2-OH were the predominant cellular fatty acids. The major polar lipids were phosphatidylethanolamine, lyso-phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The G+C content of genomic DNA was 70.4 mol%. Phylogenetic analysis using the 16S rRNA gene sequences showed that strain 201-F6T was affiliated to the genus Ideonella, and was closely related to Ideonella dechloratans LMG 28178T (97.7 %) and Ideonella azotifigens JCM 15503T (96.6 %). Strain 201-F6T could be clearly distinguished from the related species of the genus Ideonella by its physiological and biochemical characteristics as well as by its phylogenetic position and DNA-DNA relatedness. Therefore, the strain represents a novel species of the genus Ideonella, for which the name Ideonella sakaiensis sp. nov. (type strain 201-F6T=NBRC 110686T=TISTR 2288T) is proposed.
KeywordMeSH Terms
Microbial Consortia
Phylogeny
52082 Yoshida  S, Hiraga  K, Takehana  T, Taniguchi  I, Yamaji  H, Maeda  Y, Toyohara  K, Miyamoto  K, Kimura  Y, Oda  K,     ( 2016 )

Response to Comment on "A bacterium that degrades and assimilates poly(ethylene terephthalate)".

Science (New York, N.Y.) 353 (6301)
PMID : 27540160 DOI  :   10.1126/science.aaf8625    
Abstract >>
Yang et al suggest that the use of low-crystallinity poly(ethylene terephthalate) (PET) exaggerates our results. However, the primary focus of our study was identifying an organism capable of the biological degradation and assimilation of PET, regardless of its crystallinity. We provide additional PET depolymerization data that further support several other lines of data showing PET assimilation by growing cells of Ideonella sakaiensis.
KeywordMeSH Terms

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