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1. Harreither  W, Sygmund  C, Augustin  M, Narciso  M, Rabinovich  ML, Gorton  L, Haltrich  D, Ludwig  R,     ( 2011 )

Catalytic properties and classification of cellobiose dehydrogenases from ascomycetes.

Applied and environmental microbiology 77 (5)
PMID : 21216904  :   DOI  :   10.1128/AEM.02052-10     PMC  :   PMC3067291    
Abstract >>
Putative cellobiose dehydrogenase (CDH) genes are frequently discovered in various fungi by genome sequencing projects. The expression of CDH, an extracellular flavocytochrome, is well studied in white rot basidiomycetes and is attributed to extracellular lignocellulose degradation. CDH has also been reported for plant-pathogenic or saprotrophic ascomycetes, but the molecular and catalytic properties of these enzymes are currently less investigated. This study links various ascomycetous cdh genes with the molecular and catalytic characteristics of the mature proteins and suggests a differentiation of ascomycete class II CDHs into two subclasses, namely, class IIA and class IIB, in addition to the recently introduced class III of hypothetical ascomycete CDHs. This new classification is based on sequence and biochemical data obtained from sequenced fungal genomes and a screening of 40 ascomycetes. Thirteen strains showed CDH activity when they were grown on cellulose-based media, and Chaetomium atrobrunneum, Corynascus thermophilus, Dichomera saubinetii, Hypoxylon haematostroma, Neurospora crassa, and Stachybotrys bisbyi were selected for detailed studies. In these strains, one or two cdh-encoding genes were found that stem either from class IIA and contain a C-terminal carbohydrate-binding module or from class IIB without such a module. In several strains, both genes were found. Regarding substrate specificity, class IIB CDHs show a less pronounced substrate specificity for cellobiose than class IIA enzymes. A pH-dependent pattern of the intramolecular electron transfer was also observed, and the CDHs were classified into three groups featuring acidic, intermediate, or alkaline pH optima. The pH optimum, however, does not correlate with the CDH subclasses and is most likely a species-dependent adaptation to different habitats.
KeywordMeSH Terms
2. Wang  XW, Houbraken  J, Groenewald  JZ, Meijer  M, Andersen  B, Nielsen  KF, Crous  PW, Samson  RA,     ( 2016 )

Diversity and taxonomy of Chaetomium and chaetomium-like fungi from indoor environments.

Studies in mycology 84 (N/A)
PMID : 28082757  :   DOI  :   10.1016/j.simyco.2016.11.005     PMC  :   PMC5226397    
Abstract >>
During a study of indoor fungi, 145 isolates belonging to Chaetomiaceae were cultured from air, swab and dust samples from 19 countries. Based on the phylogenetic analyses of DNA-directed RNA polymerase II second largest subunit (rpb2), �]-tubulin (tub2), ITS and 28S large subunit (LSU) nrDNA sequences, together with morphological comparisons with related genera and species, 30 indoor taxa are recognised, of which 22 represent known species, seven are described as new, and one remains to be identified to species level. In our collection, 69 % of the indoor isolates with six species cluster with members of the Chaetomium globosum species complex, representing Chaetomium sensu stricto. The other indoor species fall into nine lineages that are separated from each other with several known chaetomiaceous genera occurring among them. No generic names are available for five of those lineages, and the following new genera are introduced here: Amesia with three indoor species, Arcopilus with one indoor species, Collariella with four indoor species, Dichotomopilus with seven indoor species and Ovatospora with two indoor species. The generic concept of Botryotrichum is expanded to include Emilmuelleria and the chaetomium-like species B. muromum (= Ch. murorum) in which two indoor species are included. The generic concept of Subramaniula is expanded to include several chaetomium-like taxa as well as one indoor species. Humicola is recognised as a distinct genus including two indoor taxa. According to this study, Ch. globosum is the most abundant Chaetomiaceae indoor species (74/145), followed by Ch. cochliodes (17/145), Ch. elatum (6/145) and B. piluliferum (5/145). The morphological diversity of indoor Chaetomiaceae as well as the morphological characteristics of the new genera are described and illustrated. This taxonomic study redefines the generic concept of Chaetomium and provides new insight into the phylogenetic relationships among different genera within Chaetomiaceae.
KeywordMeSH Terms
Amesia X. Wei Wang, Samson & Crous
Amesia atrobrunnea (Ames) X. Wei Wang & Samson
Amesia cymbiformis (Lodha) X. Wei Wang & Samson
Amesia gelasinospora (Aue & Müller) X. Wei Wang & Samson
Amesia nigricolor (Ames) X. Wei Wang & Samson
Arcopilus X. Wei Wang, Samson & Crous
Arcopilus aureus (Chivers) X. Wei Wang & Samson
Arcopilus cupreus (Ames) X. Wei Wang & Samson
Arcopilus flavigenus (van Warmelo) X. Wei Wang & Samson
Arcopilus fusiformis (Chivers) X. Wei Wang & Samson
Arcopilus turgidopilosus (Ames) X. Wei Wang & Samson
Botryotrichum murorum (Corda) X. Wei Wang & Samson
Botryotrichum spirotrichum (R.K. Benjamin) X. Wei Wang & Samson
Chaetomiaceae
Chaetomium elatum Kunze
Chaetomium tectifimeti X. Wei Wang & Samson
Collariella X. Wei Wang, Samson & Crous
Collariella bostrychodes (Zopf) X. Wei Wang & Samson
Collariella carteri X. Wei Wang, Houbraken & Samson
Collariella causiiformis (Ames) X. Wei Wang & Samson
Collariella gracilis (Udagawa) X. Wei Wang & Samson
Collariella quadrangulata (Chivers) X. Wei Wang & Samson
Collariella robusta (Ames) X. Wei Wang & Samson
Collariella virescens (Arx) X. Wei Wang & Samson
Dichotomopilus X. Wei Wang, Samson & Crous
Dichotomopilus dolichotrichus (Ames) X. Wei Wang & Samson
Dichotomopilus erectus (Skolko & J.W. Groves) X. Wei Wang & Samson
Dichotomopilus funicola (Cooke) X. Wei Wang & Samson
Dichotomopilus fusus (Ames) X. Wei Wang & Samson
Dichotomopilus indicus (Corda) X. Wei Wang & Samson
Dichotomopilus pratensis (X.W. Wang & L. Cai) X. Wei Wang & Samson
Dichotomopilus pseudoerectus X. Wei Wang & Samson
Dichotomopilus pseudofunicola X. Wei Wang & Samson
Dichotomopilus ramosissimus (X.W. Wang & L. Cai) X. Wei Wang & Samson
Dichotomopilus reflexus (Skolko & J.W. Groves) X. Wei Wang & Samson
Dichotomopilus subfunicola (X.W. Wang & L. Cai) X. Wei Wang & Samson
Dichotomopilus variostiolatus (Carter) X. Wei Wang & Samson
Humicola olivacea X. Wei Wang & Samson
Indoor species
Melanocarpus tardus X. Wei Wang & Samson
Morphological diversity
Ovatospora X. Wei Wang, Samson & Crous
Ovatospora brasiliensis (Batista & Pontual) X. Wei Wang & Samson
Ovatospora medusarum (Meyer & Lanneau) X. Wei Wang & Samson
Ovatospora mollicella (Ames) X. Wei Wang & Samson
Ovatospora pseudomollicella X. Wei Wang & Samson
Ovatospora senegalensis (Ames) X. Wei Wang & Samson
Ovatospora unipora (Aue & Müller) X. Wei Wang & Samson
Phylogeny
Subramaniula anamorphosa (S.A. Ahmed et al.) X. Wei Wang & Samson
Subramaniula cristata (Ames) X. Wei Wang & Samson
Subramaniula cuniculorum (Fuckel) X. Wei Wang & Samson
Subramaniula flavipila X. Wei Wang & Samson
Subramaniula fusispora (G. Smith) X. Wei Wang & Samson
Amesia X. Wei Wang, Samson & Crous
Amesia atrobrunnea (Ames) X. Wei Wang & Samson
Amesia cymbiformis (Lodha) X. Wei Wang & Samson
Amesia gelasinospora (Aue & Müller) X. Wei Wang & Samson
Amesia nigricolor (Ames) X. Wei Wang & Samson
Arcopilus X. Wei Wang, Samson & Crous
Arcopilus aureus (Chivers) X. Wei Wang & Samson
Arcopilus cupreus (Ames) X. Wei Wang & Samson
Arcopilus flavigenus (van Warmelo) X. Wei Wang & Samson
Arcopilus fusiformis (Chivers) X. Wei Wang & Samson
Arcopilus turgidopilosus (Ames) X. Wei Wang & Samson
Botryotrichum murorum (Corda) X. Wei Wang & Samson
Botryotrichum spirotrichum (R.K. Benjamin) X. Wei Wang & Samson
Chaetomiaceae
Chaetomium elatum Kunze
Chaetomium tectifimeti X. Wei Wang & Samson
Collariella X. Wei Wang, Samson & Crous
Collariella bostrychodes (Zopf) X. Wei Wang & Samson
Collariella carteri X. Wei Wang, Houbraken & Samson
Collariella causiiformis (Ames) X. Wei Wang & Samson
Collariella gracilis (Udagawa) X. Wei Wang & Samson
Collariella quadrangulata (Chivers) X. Wei Wang & Samson
Collariella robusta (Ames) X. Wei Wang & Samson
Collariella virescens (Arx) X. Wei Wang & Samson
Dichotomopilus X. Wei Wang, Samson & Crous
Dichotomopilus dolichotrichus (Ames) X. Wei Wang & Samson
Dichotomopilus erectus (Skolko & J.W. Groves) X. Wei Wang & Samson
Dichotomopilus funicola (Cooke) X. Wei Wang & Samson
Dichotomopilus fusus (Ames) X. Wei Wang & Samson
Dichotomopilus indicus (Corda) X. Wei Wang & Samson
Dichotomopilus pratensis (X.W. Wang & L. Cai) X. Wei Wang & Samson
Dichotomopilus pseudoerectus X. Wei Wang & Samson
Dichotomopilus pseudofunicola X. Wei Wang & Samson
Dichotomopilus ramosissimus (X.W. Wang & L. Cai) X. Wei Wang & Samson
Dichotomopilus reflexus (Skolko & J.W. Groves) X. Wei Wang & Samson
Dichotomopilus subfunicola (X.W. Wang & L. Cai) X. Wei Wang & Samson
Dichotomopilus variostiolatus (Carter) X. Wei Wang & Samson
Humicola olivacea X. Wei Wang & Samson
Indoor species
Melanocarpus tardus X. Wei Wang & Samson
Morphological diversity
Ovatospora X. Wei Wang, Samson & Crous
Ovatospora brasiliensis (Batista & Pontual) X. Wei Wang & Samson
Ovatospora medusarum (Meyer & Lanneau) X. Wei Wang & Samson
Ovatospora mollicella (Ames) X. Wei Wang & Samson
Ovatospora pseudomollicella X. Wei Wang & Samson
Ovatospora senegalensis (Ames) X. Wei Wang & Samson
Ovatospora unipora (Aue & Müller) X. Wei Wang & Samson
Phylogeny
Subramaniula anamorphosa (S.A. Ahmed et al.) X. Wei Wang & Samson
Subramaniula cristata (Ames) X. Wei Wang & Samson
Subramaniula cuniculorum (Fuckel) X. Wei Wang & Samson
Subramaniula flavipila X. Wei Wang & Samson
Subramaniula fusispora (G. Smith) X. Wei Wang & Samson
Subramaniula fusispora (G. Smith) X. Wei Wang & Samson
Amesia X. Wei Wang, Samson & Crous
Amesia atrobrunnea (Ames) X. Wei Wang & Samson
Amesia cymbiformis (Lodha) X. Wei Wang & Samson
Amesia gelasinospora (Aue & Müller) X. Wei Wang & Samson
Amesia nigricolor (Ames) X. Wei Wang & Samson
Arcopilus X. Wei Wang, Samson & Crous
Arcopilus aureus (Chivers) X. Wei Wang & Samson
Arcopilus cupreus (Ames) X. Wei Wang & Samson
Arcopilus flavigenus (van Warmelo) X. Wei Wang & Samson
Arcopilus fusiformis (Chivers) X. Wei Wang & Samson
Arcopilus turgidopilosus (Ames) X. Wei Wang & Samson
Botryotrichum murorum (Corda) X. Wei Wang & Samson
Botryotrichum spirotrichum (R.K. Benjamin) X. Wei Wang & Samson
Chaetomiaceae
Chaetomium elatum Kunze
Chaetomium tectifimeti X. Wei Wang & Samson
Collariella X. Wei Wang, Samson & Crous
Collariella bostrychodes (Zopf) X. Wei Wang & Samson
Collariella carteri X. Wei Wang, Houbraken & Samson
Collariella causiiformis (Ames) X. Wei Wang & Samson
Collariella gracilis (Udagawa) X. Wei Wang & Samson
Collariella quadrangulata (Chivers) X. Wei Wang & Samson
Collariella robusta (Ames) X. Wei Wang & Samson
Collariella virescens (Arx) X. Wei Wang & Samson
Dichotomopilus X. Wei Wang, Samson & Crous
Dichotomopilus dolichotrichus (Ames) X. Wei Wang & Samson
Dichotomopilus erectus (Skolko & J.W. Groves) X. Wei Wang & Samson
Dichotomopilus funicola (Cooke) X. Wei Wang & Samson
Dichotomopilus fusus (Ames) X. Wei Wang & Samson
Dichotomopilus indicus (Corda) X. Wei Wang & Samson
Dichotomopilus pratensis (X.W. Wang & L. Cai) X. Wei Wang & Samson
Dichotomopilus pseudoerectus X. Wei Wang & Samson
Dichotomopilus pseudofunicola X. Wei Wang & Samson
Dichotomopilus ramosissimus (X.W. Wang & L. Cai) X. Wei Wang & Samson
Dichotomopilus reflexus (Skolko & J.W. Groves) X. Wei Wang & Samson
Dichotomopilus subfunicola (X.W. Wang & L. Cai) X. Wei Wang & Samson
Dichotomopilus variostiolatus (Carter) X. Wei Wang & Samson
Humicola olivacea X. Wei Wang & Samson
Indoor species
Melanocarpus tardus X. Wei Wang & Samson
Morphological diversity
Ovatospora X. Wei Wang, Samson & Crous
Ovatospora brasiliensis (Batista & Pontual) X. Wei Wang & Samson
Ovatospora medusarum (Meyer & Lanneau) X. Wei Wang & Samson
Ovatospora mollicella (Ames) X. Wei Wang & Samson
Ovatospora pseudomollicella X. Wei Wang & Samson
Ovatospora senegalensis (Ames) X. Wei Wang & Samson
Ovatospora unipora (Aue & Müller) X. Wei Wang & Samson
Phylogeny
Subramaniula anamorphosa (S.A. Ahmed et al.) X. Wei Wang & Samson
Subramaniula cristata (Ames) X. Wei Wang & Samson
Subramaniula cuniculorum (Fuckel) X. Wei Wang & Samson
Subramaniula flavipila X. Wei Wang & Samson
Amesia X. Wei Wang, Samson & Crous
Amesia atrobrunnea (Ames) X. Wei Wang & Samson
Amesia cymbiformis (Lodha) X. Wei Wang & Samson
Amesia gelasinospora (Aue & Müller) X. Wei Wang & Samson
Amesia nigricolor (Ames) X. Wei Wang & Samson
Arcopilus X. Wei Wang, Samson & Crous
Arcopilus aureus (Chivers) X. Wei Wang & Samson
Arcopilus cupreus (Ames) X. Wei Wang & Samson
Arcopilus flavigenus (van Warmelo) X. Wei Wang & Samson
Arcopilus fusiformis (Chivers) X. Wei Wang & Samson
Arcopilus turgidopilosus (Ames) X. Wei Wang & Samson
Botryotrichum murorum (Corda) X. Wei Wang & Samson
Botryotrichum spirotrichum (R.K. Benjamin) X. Wei Wang & Samson
Chaetomiaceae
Chaetomium elatum Kunze
Chaetomium tectifimeti X. Wei Wang & Samson
Collariella X. Wei Wang, Samson & Crous
Collariella bostrychodes (Zopf) X. Wei Wang & Samson
Collariella carteri X. Wei Wang, Houbraken & Samson
Collariella causiiformis (Ames) X. Wei Wang & Samson
Collariella gracilis (Udagawa) X. Wei Wang & Samson
Collariella quadrangulata (Chivers) X. Wei Wang & Samson
Collariella robusta (Ames) X. Wei Wang & Samson
Collariella virescens (Arx) X. Wei Wang & Samson
Dichotomopilus X. Wei Wang, Samson & Crous
Dichotomopilus dolichotrichus (Ames) X. Wei Wang & Samson
Dichotomopilus erectus (Skolko & J.W. Groves) X. Wei Wang & Samson
Dichotomopilus funicola (Cooke) X. Wei Wang & Samson
Dichotomopilus fusus (Ames) X. Wei Wang & Samson
Dichotomopilus indicus (Corda) X. Wei Wang & Samson
Dichotomopilus pratensis (X.W. Wang & L. Cai) X. Wei Wang & Samson
Dichotomopilus pseudoerectus X. Wei Wang & Samson
Dichotomopilus pseudofunicola X. Wei Wang & Samson
Dichotomopilus ramosissimus (X.W. Wang & L. Cai) X. Wei Wang & Samson
Dichotomopilus reflexus (Skolko & J.W. Groves) X. Wei Wang & Samson
Dichotomopilus subfunicola (X.W. Wang & L. Cai) X. Wei Wang & Samson
Dichotomopilus variostiolatus (Carter) X. Wei Wang & Samson
Humicola olivacea X. Wei Wang & Samson
Indoor species
Melanocarpus tardus X. Wei Wang & Samson
Morphological diversity
Ovatospora X. Wei Wang, Samson & Crous
Ovatospora brasiliensis (Batista & Pontual) X. Wei Wang & Samson
Ovatospora medusarum (Meyer & Lanneau) X. Wei Wang & Samson
Ovatospora mollicella (Ames) X. Wei Wang & Samson
Ovatospora pseudomollicella X. Wei Wang & Samson
Ovatospora senegalensis (Ames) X. Wei Wang & Samson
Ovatospora unipora (Aue & Müller) X. Wei Wang & Samson
Phylogeny
Subramaniula anamorphosa (S.A. Ahmed et al.) X. Wei Wang & Samson
Subramaniula cristata (Ames) X. Wei Wang & Samson
Subramaniula cuniculorum (Fuckel) X. Wei Wang & Samson
Subramaniula flavipila X. Wei Wang & Samson
Subramaniula fusispora (G. Smith) X. Wei Wang & Samson
Amesia X. Wei Wang, Samson & Crous
Amesia atrobrunnea (Ames) X. Wei Wang & Samson
Amesia cymbiformis (Lodha) X. Wei Wang & Samson
Amesia gelasinospora (Aue & Müller) X. Wei Wang & Samson
Amesia nigricolor (Ames) X. Wei Wang & Samson
Arcopilus X. Wei Wang, Samson & Crous
Arcopilus aureus (Chivers) X. Wei Wang & Samson
Arcopilus cupreus (Ames) X. Wei Wang & Samson
Arcopilus flavigenus (van Warmelo) X. Wei Wang & Samson
Arcopilus fusiformis (Chivers) X. Wei Wang & Samson
Arcopilus turgidopilosus (Ames) X. Wei Wang & Samson
Botryotrichum murorum (Corda) X. Wei Wang & Samson
Botryotrichum spirotrichum (R.K. Benjamin) X. Wei Wang & Samson
Chaetomiaceae
Chaetomium elatum Kunze
Chaetomium tectifimeti X. Wei Wang & Samson
Collariella X. Wei Wang, Samson & Crous
Collariella bostrychodes (Zopf) X. Wei Wang & Samson
Collariella carteri X. Wei Wang, Houbraken & Samson
Collariella causiiformis (Ames) X. Wei Wang & Samson
Collariella gracilis (Udagawa) X. Wei Wang & Samson
Collariella quadrangulata (Chivers) X. Wei Wang & Samson
Collariella robusta (Ames) X. Wei Wang & Samson
Collariella virescens (Arx) X. Wei Wang & Samson
Dichotomopilus X. Wei Wang, Samson & Crous
Dichotomopilus dolichotrichus (Ames) X. Wei Wang & Samson
Dichotomopilus erectus (Skolko & J.W. Groves) X. Wei Wang & Samson
Dichotomopilus funicola (Cooke) X. Wei Wang & Samson
Dichotomopilus fusus (Ames) X. Wei Wang & Samson
Dichotomopilus indicus (Corda) X. Wei Wang & Samson
Dichotomopilus pratensis (X.W. Wang & L. Cai) X. Wei Wang & Samson
Dichotomopilus pseudoerectus X. Wei Wang & Samson
Dichotomopilus pseudofunicola X. Wei Wang & Samson
Dichotomopilus ramosissimus (X.W. Wang & L. Cai) X. Wei Wang & Samson
Dichotomopilus reflexus (Skolko & J.W. Groves) X. Wei Wang & Samson
Dichotomopilus subfunicola (X.W. Wang & L. Cai) X. Wei Wang & Samson
Dichotomopilus variostiolatus (Carter) X. Wei Wang & Samson
Humicola olivacea X. Wei Wang & Samson
Indoor species
Melanocarpus tardus X. Wei Wang & Samson
Morphological diversity
Ovatospora X. Wei Wang, Samson & Crous
Ovatospora brasiliensis (Batista & Pontual) X. Wei Wang & Samson
Ovatospora medusarum (Meyer & Lanneau) X. Wei Wang & Samson
Ovatospora mollicella (Ames) X. Wei Wang & Samson
Ovatospora pseudomollicella X. Wei Wang & Samson
Ovatospora senegalensis (Ames) X. Wei Wang & Samson
Ovatospora unipora (Aue & Müller) X. Wei Wang & Samson
Phylogeny
Subramaniula anamorphosa (S.A. Ahmed et al.) X. Wei Wang & Samson
Subramaniula cristata (Ames) X. Wei Wang & Samson
Subramaniula cuniculorum (Fuckel) X. Wei Wang & Samson
Subramaniula flavipila X. Wei Wang & Samson
Subramaniula fusispora (G. Smith) X. Wei Wang & Samson
3. Busk  PK, Lange  L,     ( 2013 )

Cellulolytic potential of thermophilic species from four fungal orders.

AMB Express 3 (1)
PMID : 23958135  :   DOI  :   10.1186/2191-0855-3-47     PMC  :   PMC3766086    
Abstract >>
Elucidation of fungal biomass degradation is important for understanding the turnover of biological materials in nature and has important implications for industrial biomass conversion. In recent years there has been an increasing interest in elucidating the biological role of thermophilic fungi and in characterization of their industrially useful enzymes. In the present study we investigated the cellulolytic potential of 16 thermophilic fungi from the three ascomycete orders Sordariales, Eurotiales and Onygenales and from the zygomycete order Mucorales thus covering all fungal orders that include thermophiles. Thermophilic fungi are the only described eukaryotes that can grow at temperatures above 45�XC. All 16 fungi were able to grow on crystalline cellulose but their secreted enzymes showed widely different cellulolytic activities, pH optima and thermostabilities. Interestingly, in contrast to previous reports, we found that some fungi such as Melanocarpus albomyces readily grew on crystalline cellulose and produced cellulases. These results indicate that there are large differences in the cellulolytic potential of different isolates of the same species. Furthermore, all the selected species were able to degrade cellulose but the differences in cellulolytic potential and thermostability of the secretome did not correlate to the taxonomic position. PCR amplification and sequencing of 22 cellulase genes from the fungi showed that the level of thermostability of the cellulose-degrading activity could not be inferred from the phylogenetic relationship of the cellulases.
KeywordMeSH Terms
4.     ( 2013 )

Function-based classification of carbohydrate-active enzymes by recognition of short, conserved peptide motifs.

Applied and environmental microbiology 79 (11)
PMID : 23524681  :   DOI  :   10.1128/AEM.03803-12     PMC  :   PMC3648057    
Abstract >>
Functional prediction of carbohydrate-active enzymes is difficult due to low sequence identity. However, similar enzymes often share a few short motifs, e.g., around the active site, even when the overall sequences are very different. To exploit this notion for functional prediction of carbohydrate-active enzymes, we developed a simple algorithm, peptide pattern recognition (PPR), that can divide proteins into groups of sequences that share a set of short conserved sequences. When this method was used on 118 glycoside hydrolase 5 proteins with 9% average pairwise identity and representing four characterized enzymatic functions, 97% of the proteins were sorted into groups correlating with their enzymatic activity. Furthermore, we analyzed 8,138 glycoside hydrolase 13 proteins including 204 experimentally characterized enzymes with 28 different functions. There was a 91% correlation between group and enzyme activity. These results indicate that the function of carbohydrate-active enzymes can be predicted with high precision by finding short, conserved motifs in their sequences. The glycoside hydrolase 61 family is important for fungal biomass conversion, but only a few proteins of this family have been functionally characterized. Interestingly, PPR divided 743 glycoside hydrolase 61 proteins into 16 subfamilies useful for targeted investigation of the function of these proteins and pinpointed three conserved motifs with putative importance for enzyme activity. Furthermore, the conserved sequences were useful for cloning of new, subfamily-specific glycoside hydrolase 61 proteins from 14 fungi. In conclusion, identification of conserved sequence motifs is a new approach to sequence analysis that can predict carbohydrate-active enzyme functions with high precision.
KeywordMeSH Terms
Algorithms

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