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Taxonomy Citation ID Reference
41353 Sheu  SY, Chiu  TF, Young  CC, Arun  AB, Chen  WM,     ( 2011 )

Flavobacterium macrobrachii sp. nov., isolated from a freshwater shrimp culture pond.

International journal of systematic and evolutionary microbiology 61 (Pt 6)
PMID : 20601490 DOI  :   10.1099/ijs.0.025403-0    
Abstract >>
A bacterial strain, designated an-8(T), was isolated from a freshwater shrimp culture pond in Taiwan and characterized using a polyphasic taxonomic approach. Cells of strain an-8(T) were Gram-reaction-negative, aerobic, rod-shaped and non-motile, formed yellow-pigmented colonies and grew at 15-30 �XC (optimum 25 �XC), pH 7-8 (optimum pH 8.0) and in 0-1 % (w/v) NaCl (optimum 0 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain an-8(T) belonged to the genus Flavobacterium and its most closely related neighbours were Flavobacterium terrigena DS-20(T) and Flavobacterium terrae R2A1-13(T) with sequence similarities of 95.1 and 94.9 %, respectively. Strain an-8(T) contained iso-C(15 : 0), summed feature 3 (C(16 : 1)�s6c and/or C(16 : 1)�s7c), iso-C(16 : 0) 3-OH, iso-C(15 : 0) 3-OH, iso-C(17 : 0) 3-OH and iso-C(15 : 1) as the major fatty acids. The major isoprenoid quinone was MK-6. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidyldimethylethanolamine, phosphatidylserine and several unidentified polar lipids. The G+C content of the genomic DNA was 39.8 mol%. On the basis of the phylogenetic and phenotypic data, strain an-8(T) represents a novel species of the genus Flavobacterium, for which the name Flavobacterium macrobrachii sp. nov. is proposed. The type strain is an-8(T) (= BCRC 17965(T) = LMG 25203(T)).
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