| Taxonomy Citation ID | Reference | ||||
|---|---|---|---|---|---|
| 12893 |
Masco L,
Ventura M,
Zink R,
Huys G,
Swings J,
( 2004 ) Polyphasic taxonomic analysis of Bifidobacterium animalis and Bifidobacterium lactis reveals relatedness at the subspecies level: reclassification of Bifidobacterium animalis as Bifidobacterium animalis subsp. animalis subsp. nov. and Bifidobacterium lactis as Bifidobacterium animalis subsp. lactis subsp. nov. PMID : 15280282 DOI : 10.1099/ijs.0.03011-0 Abstract >>
The taxonomic standing of Bifidobacterium lactis and Bifidobacterium animalis was investigated using a polyphasic approach. Sixteen representatives of both taxa were found to be phenotypically similar and shared more than 70% DNA-DNA relatedness (76-100%), which reinforces the conclusions of previous studies in which B. lactis and B. animalis were considered to be one single species. However, the results of protein profiling, BOX-PCR fingerprinting, Fluorescent Amplified Fragment Length Polymorphism (FAFLP), and atpD and groEL gene sequence analysis demonstrate that representatives of B. animalis and B. lactis constitute two clearly separated subgroups; this subdivision was also phenotypically supported based on the ability to grow in milk. Given the fact that B. lactis Meile et al. 1997 has to be considered as a junior synonym of B. animalis (Mitsuoka 1969) Scardovi and Trovatelli 1974, our data indicate that the latter species should be split into two new subspecies, i.e. Bifidobacterium animalis subsp. animalis subsp. nov. (type strain R101-8T=LMG 10508T=ATCC 25527T=DSM 20104T=JCM 1190T) and Bifidobacterium animalis subsp. lactis subsp. nov. (type strain UR1T=LMG 18314T=DSM 10140T=JCM 10602T).
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11377 |
Ventura M,
Zink R,
( 2002 ) Rapid identification, differentiation, and proposed new taxonomic classification of Bifidobacterium lactis. PMID : 12450873 DOI : 10.1128/aem.68.12.6429-6434.2002 PMC : PMC134397 Abstract >>
Identification of Bifidobacterium lactis and Bifidobacterium animalis is problematic because of phenotypic and genetic homogeneities and has raised the question of whether they belong to one unique taxon. Analysis of the 16S-23S internally transcribed spacer region of B. lactis DSM10140(T), B. animalis ATCC 25527(T), and six potential B. lactis strains suggested two distinct clusters. Two specific 16S-23S spacer rRNA gene-targeted primers have been developed for specific detection of B. animalis. All of the molecular techniques used (B. lactis or B. animalis PCR primers, enterobacterial repetitive intergenic consensus PCR) demonstrated that B. lactis and B. animalis form two main groups and suggest a revision of the strains assigned to B. animalis. We propose that B. lactis should be separated from B. animalis at the subspecies level.
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| 8925 | VALIDATION LIST No. 62. Int. J. Syst. Bacteriol. (1997) 47:915-916. | ||||
| 8924 | Klein, G. "International Committee on Systematic Bacteriology. Subcommittee on the taxonomy of Bifidobacterium, Lactobacillus and related organisms. Minutes of the meetings, 22 and 23 September 1999, Veldhoven, The Netherlands." Int. J. Syst. Evol. Microbiol. (2001) 51:259-261. [No PubMed record available.] | 3541 |
Cai Y,
Matsumoto M,
Benno Y,
( 2000 ) Bifidobacterium lactis Meile et al. 1997 is a subjective synonym of Bifidobacterium animalis (Mitsuoka 1969) Scardovi and Trovatelli 1974. PMID : 11128064 DOI : 10.1111/j.1348-0421.2000.tb02568.x Abstract >>
Bifidobacterium lactis JCM 10602T (T = type strain) and Bifidobacterium animalis JCM 1190T were found to be phenotypically similar. These strains were subjected to investigation of their genetic relationships. The 16S rRNA sequence of B. animalis JCM 1190T was aligned with that of other Bifidobacterium species. B. animalis and B. lactis were the most closely related species in the phylogenetic tree and showed a high similarity in sequences (98.8%). The levels of DNA-DNA hybridization between the type strains of B. lactis and B. animalis ranged from 85.5 to 92.3%, showing that they represent a single species. It is proposed that B. lactis should be considered as a junior subjective synonym of B. animalis.
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| 8926 | Scardovi, V., and Trovatelli, L.D. "Bifidobacterium animalis (Mitsuoka) comb. nov. and the 'minimum' and 'subtile' groups of new bifidobacteria found in sewage." Int. J. Syst. Bacteriol. (1974) 24:21-28. [No PubMed record available.] | ||||
| 8923 | Mitsuoka, T. "Vergleichende Untersuchungen uber die Bifidobakterien aus dem Berdauungstrakt von Menschen und Tieren." Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. I (1969) 210:52-64. [No PubMed record available.] |
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